Cytiva HiTrap™ NHS-Activated HP Columns

Couple ligands containing primary amines easily. Cytiva Life Sciences™ HiTrap™ NHS-Activated HP Columns are prepacked with NHS activated Sepharose™ High Performance.

Includes: Complete instructions for use and method optimization, plus a variety of connectors for syringe, peristaltic pump, and chromatography systems

Catalog Number	Mfr. No.	Bed Volume	Diameter (Metric) Inner	Flow Rate	Quantity
45-000-137	Cytiva 17071601	1mL	0.7cm	4 mL/min.	5 x 1mL
45-000-138	Cytiva 17071701	5mL	1.6cm	20 mL/min.	1 x 5mL

Description

Prepacked with NHS-activated Sepharose™ High Performance Media for coupling ligand via primary amines at close to neutral pH

• Designed for use with a syringe, peristaltic pump, or chromatography system
• Degree of substitution: 10 μmol NHS/mL medium
• Delivered in 100% isopropanol to protect the active groups
• Stable in the pH range 3 to 12, depending upon ligand
• Average particle size: 34μm

Specifications

• Particle Size: 34 μm
• Product Type: NHS-Activated HP Column
• Height (English): 0.98 in.
• Ligand Type: 10μM NHS groups/mL medium
• Max. Pressure: 42psi
• Support Type: 6% Highly Cross-linked Agarose

• For Use With (Equipment): Syringe, peristaltic pump or liquid chromato graphy system such as AKTA™ design or FPLC system
• For Use With (Application): Preparative affinity chromato graphy, easy coupling of ligands containing primary Amines
• Height (Metric): 2.5cm
• Material: Polypropylene
• pH Range: 3 to 12
• Content And Storage: 4° to 8°C