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GE Healthcare illustra™ Hot Start Master Mix

A ready-to-use 2X mastermix providing robust and reliable performance for demanding PCR applications in which high specificity and high sensitivity are essential to success

Manufacturer:  GE Healthcare 25150001

Catalog No. 46-001-001


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Description

Description

  • Convenient, ready-to-use mix saves time and reduces experimental variability
  • For any standard PCR reaction, such as multiplex PCR, and reactions exhibiting nonspecific amplification or primer-dimer formation
  • Primer sequestration effectively reduces any nonspecific priming or primer-dimer formation
  • Recombinant activator protein avoids the risk of contamination from a mammalian source associated with hot start antibodies
  • Avoids the risk of contamination from a mammalian source associated with hot start antibodies
  • Quick hot start activation preserves sample integrity by avoiding extensive precycling heat treatment

Specifications

Specifications

Hot Start Master Mix is a 2X premixed formulation that can effectively reduce nonspecific priming and primer-dimer formation during PCR.
Solution
100.0
-20°C
For PCR application
2X Concentrate solution of 20mM Tris HCl (pH 8.6), 100mM KCl, 3mM MgCl2, 0.4mM dNTPs (dATP, dCTP, dGTP, dTTP), 50U/mL Taq DNA Polymerase, hot start activator protein, stabilizers
100 Reactions
50μL
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