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GE Healthcare Sephadex™ G50 Superfine Media

Use as a well established gel filtration medium for desalting and buffer exchange of biomolecules >30 000 molecular weight. Sephadex™ G50 Superfine Media's small bead size gives higher efficiency.

Manufacturer:  GE Healthcare 17004101

Catalog No. 45-000-015


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Description

Description

  • For purposes of determining maximum flowrate, beads behave as rigid spheres according to Darcy's Law
  • Working pH range 2 to 10
  • Particle size range for wet beads 20 to 80µm
  • Bed volume in distilled water 9 to 11mL/g
  • Fractionation ranges: 5 x 102 to 1 x 104 for dextrans, 1.5 x 103 to 3 x 104 for globular proteins

Specifications

Specifications

100g
< Darcy´s Law
Sephadex G-50 Superfine, 100 g
5cm
3.93 in.
min 60 cm/h, pressure drop cm H2O/bed height=15, bed height 10 cm, column 5 cm i.d.
2 to 10
DNA
4 to 30°C
9 to 11mg/g
Solid Bead
Purification of DNA from small molecules by Gel filtration (higher efficiency with shorter diffusion distances)
10cm
20 to 80μm in wet form, 20 to 50μm in dry form
Cross-linked Dextran
Documents
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