Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More
  1. Home
  2. Products
  3. Cell Lines and Blood Products
  4. Cell Lines and Cell Cultures
  5. General Cell Lines and Cell Cultures
  6. K1688

Thermo Scientific™ GeneBLAzer™ ERα-UAS-bla GripTite™ Cells

Catalog No. K1688
Encompass_Preferred
Change view
Click to view available options
Quantity:
2 x 106 cells
1 product options available for selection
Product selection table with 1 available options. Use arrow keys to navigate and Enter or Space to select.
Catalog No. Quantity
K1688 2 x 106 cells
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 1 options available.
1 options
Catalog No. K1688 Supplier Thermo Scientific™ Supplier No. K1688
Only null left
Add to Cart
Add to Cart
Request a Quote

Description

ER alpha-UAS-bla GripTite™cells contain the ligand-binding domain (LBD) of the human Estrogen receptor alpha (ER alpha) fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer™ UAS-bla GripTite™ cell line.

ER alpha-UAS-bla GripTite™cells contain the ligand-binding domain (LBD) of the human Estrogen receptor alpha (ER alpha) fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer™ UAS-bla GripTite™ cell line. GeneBLAzer™ UAS-bla GripTite™ cells stably express a beta-lactamase reporter gene under the transcriptional control of an upstream activator sequence (UAS). When an agonist binds to the LBD of the GAL4 (DBD)-ER alpha (LBD) fusion protein, the protein binds to the UAS, resulting in expression of beta-lactamase. Division Arrested (DA) cells are available in two configurations- an Assay Kit (which includes cells and sufficient substrate to analyze 1 x 384-well plate), and a tube of cells sufficient to analyze 10 x 384-well plates. ER alpha-UAS-bla GripTite™ cells are functionally validated for Z' and EC50 concentrations of 17-beta Estradiol. In addition, ER alpha-UAS-bla GripTite™ cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, stimulation time, and substrate loading time (data available upon request). Additional testing data using alternate stimuli are also available.

TRUSTED_SUSTAINABILITY

Specifications

Alternate Agonists Betamethasone, Cortisol, Budesonide
Antagonists Milfepristone, Cortisone, Progesterone
Assay Entry Cell-based beta lactamase reporter gene
Cell State Dividing Cells
Content And Storage ER alpha-UAS-bla GriptTite™ cells (K1090)

Shipping: Dry Ice
Storage: -80°C (liquid nitrogen)
Detection Method Fluorescence
Species Human
Druggable Target Nuclear Receptors
No. of Cells 2 x 106 Cells
Primary Agonist Dexamethasone
Product Line GeneBLAzer
Product Type GripTite Cells
Quantity 2 x 106 cells
Reporter Gene BLA (Beta-Lactamase)
Shipping Condition Room Temperature
Target Entry ER alpha, ESR1, ERA, NR3A1
Show More Show Less

For Research Use Only. Not for use in diagnostic procedures.

Product Title
Select an issue

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.