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Thermo Scientific™ GeneBLAzer™ ERα-UAS-bla GripTite™ Cells

Catalog No. K1688
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Quantity:
2 x 106 cells

Description

ER alpha-UAS-bla GripTite™cells contain the ligand-binding domain (LBD) of the human Estrogen receptor alpha (ER alpha) fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer™ UAS-bla GripTite™ cell line.

ER alpha-UAS-bla GripTite™cells contain the ligand-binding domain (LBD) of the human Estrogen receptor alpha (ER alpha) fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer™ UAS-bla GripTite™ cell line. GeneBLAzer™ UAS-bla GripTite™ cells stably express a beta-lactamase reporter gene under the transcriptional control of an upstream activator sequence (UAS). When an agonist binds to the LBD of the GAL4 (DBD)-ER alpha (LBD) fusion protein, the protein binds to the UAS, resulting in expression of beta-lactamase. Division Arrested (DA) cells are available in two configurations- an Assay Kit (which includes cells and sufficient substrate to analyze 1 x 384-well plate), and a tube of cells sufficient to analyze 10 x 384-well plates. ER alpha-UAS-bla GripTite™ cells are functionally validated for Z' and EC50 concentrations of 17-beta Estradiol. In addition, ER alpha-UAS-bla GripTite™ cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, stimulation time, and substrate loading time (data available upon request). Additional testing data using alternate stimuli are also available.

TRUSTED_SUSTAINABILITY

Specifications

Alternate Agonists Betamethasone, Cortisol, Budesonide
Antagonists Milfepristone, Cortisone, Progesterone
Assay Entry Cell-based beta lactamase reporter gene
Cell State Dividing Cells
Content And Storage ER alpha-UAS-bla GriptTite™ cells (K1090)

Shipping: Dry Ice
Storage: -80°C (liquid nitrogen)
Detection Method Fluorescence
Species Human
Druggable Target Nuclear Receptors
No. of Cells 2 x 106 Cells
Primary Agonist Dexamethasone
Product Line GeneBLAzer™
Product Type GripTite Cells
Quantity 2 x 106 cells
Reporter Gene BLA (Beta-Lactamase)
Shipping Condition Room Temperature
Target Entry ER alpha, ESR1, ERA, NR3A1
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For Research Use Only. Not for use in diagnostic procedures.

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