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Thermo Scientific™ GeneBLAzer™ FXR-UAS-bla HEK 293T Cells

Catalog No. K1691B
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K1691B 2 x 106 cells
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Catalog No. K1691B Supplier Thermo Scientific™ Supplier No. K1691B
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Description

FXR-UAS-bla HEK 293T 293 cells contain the ligand-binding domain (LBD) of the human farnesoid X receptor (FXR) fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer™UAS-bla HEK 293T cell line.

GeneBLAzer™UASbla HEK 293T cells stably express a beta-lactamase reporter gene under the transcriptional control of an upstream activator sequence (UAS). When an agonist binds to the LBD of the GAL4 (DBD)-FXR (LBD) fusion protein, the protein binds to the UAS, resulting in expression of beta-lactamase. Division Arrested (DA) cells are available in two configurations- an Assay Kit (which includes cells and sufficient substrate to analyze 1 x 384-well plate), and a tube of cells sufficient to analyze 10 x 384-well plates. FXR-UAS-bla HEK 293T cells are functionally validated for Z' and EC50 concentrations of chenodeoxycholic acid (CDCA). In addition, FXR-UAS-bla HEK 293T cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, stimulation time, and substrate loading time (data available upon request). Additional testing data using alternate stimuli are also available.

TRUSTED_SUSTAINABILITY

Specifications

Cell State Dividing Cells
Content And Storage Shipping: Dry IceStorage: -80°C/Liquid NitrogenContains: FXR-UAS-bla HEK 293 T cells (K1239)
Detection Method Fluorescence
Druggable Target Nuclear Receptors
Format 384-well plate, Tube
Product Line GeneBLAzer
Product Type Cell Line
Quantity 2 x 106 cells
Reporter Gene BLA (Beta-Lactamase)

For Research Use Only. Not for use in diagnostic procedures.

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