Thermo Scientific™ GeneBLAzer™ PPARγ-UAS-bla HEK 293H Cells

Description

PPAR gamma-UAS-bla HEK 293H cells contain the ligand-binding domain (LBD) of the human Peroxisome Proliferator-Activated Receptor-gamma (PPAR gamma) fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer™UAS-bla HEK 293H cell line.

PPAR gamma-UAS-bla HEK 293H cells contain the ligand-binding domain (LBD) of the human Peroxisome Proliferator-Activated Receptor-gamma (PPAR gamma) fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer™UAS-bla HEK 293H cell line. GeneBLAzer™UAS-bla HEK 293H cells stably express a beta-lactamase reporter gene under the transcriptional control of an upstream activator sequence (UAS). When an agonist binds to the LBD of the GAL4 (DBD)-PPAR gamma (LBD) fusion protein, the protein binds to the UAS, resulting in expression of beta-lactamase. Division Arrested (DA) cells are available in two configurations- an Assay Kit (which includes cells and sufficient substrate to analyze 1 x 384-well plate), and a tube of cells sufficient to analyze 10 x 384-well plates. PPAR gamma-UAS-bla HEK 293H cells are functionally validated for Z' and EC₅₀ concentrations of Rosiglitazone. In addition, PPAR gamma-UAS-bla HEK 293H cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, and stimulation time.

Specifications

• Assay Entry: Cell-based beta lactamase reporter gene
• Cell State: Dividing Cells
• Content And Storage: PPAR gamma-UAS-bla 293H cells (K1094)
  
  Shipping: Dry Ice
  Storage: -80°C (liquid nitrogen)
• Detection Method: Fluorescence
• Species: Human
• Druggable Target: Nuclear Receptors
• Primary Agonist: Calcitriol
• Product Line: GeneBLAzer™
• Product Type: HEK 293H Cells
• Quantity: 2 x 10⁶ Cells
• Reporter Gene: BLA (Beta-Lactamase)
• Target Entry: PPARG, PPAR gamma, NR1C3

For Research Use Only. Not for use in diagnostic procedures.