Thermo Scientific™ GeneBLAzer™ RARβ-UAS-bla HEK 293T Cells

Description

RAR beta-UAS-bla HEK 293T cells contain the ligand-binding domain (LBD) of the human retinoic acid receptor beta (RAR beta) fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer™UAS-bla HEK 293T cell line.

RAR beta-UAS-bla HEK 293T cells contain the ligand-binding domain (LBD) of the human retinoic acid receptor beta (RAR beta) fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer™UAS-bla HEK 293T cell line. GeneBLAzer™UAS-bla HEK 293T cells stably express a beta-lactamase reporter gene under the transcriptional control of an upstream activator sequence (UAS). When an agonist binds to the LBD of the GAL4 (DBD)-RAR beta (LBD) fusion protein, the protein binds to the UAS, resulting in expression of beta-lactamase. RAR beta-UAS-bla HEK 293T cells are functionally validated for Z' and EC₅₀ concentrations of all-trans retinoic acid (ATRA). In addition, RAR beta-UAS-bla HEK 293T cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, and stimulation time.

Specifications

• Alternate Agonists: Docosahexaenoic Acid, Phytanic Acid, All-trans-retinoic acid
• Antagonists: none
• Assay Entry: Cell-based beta lactamase reporter gene
• Cell State: Dividing Cells
• Content And Storage: GeneBLAzer™ Nuclear Receptor Cell Lines are shipped on dry ice. Store in liquid nitrogen immediately upon receipt, or thaw for immediate use.
• Detection Method: Fluorescence
• Druggable Target: Nuclear Receptors
• No. of Cells: 2 x 10⁶
• Primary Agonist: 9-cis-retinoic acid
• Product Line: GeneBLAzer™
• Product Type: HEK 293T Cells
• Quantity: 2 x 10⁶ cells
• Reporter Gene: BLA (Beta-Lactamase)
• Target Entry: RAR beta, NR1B2, RARB

For Research Use Only. Not for use in diagnostic procedures.