Thermo Scientific™ GeneBLAzer™ RXRβ-UAS-bla HEK 293T Cells

Description

RXR beta-UAS-bla HEK 293T cells contain the ligand-binding domain (LBD) of the human Retinoid X Receptor beta (RXR beta) fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer™UAS-bla HEK 293T cell line.

RXR beta-UAS-bla HEK 293T cells contain the ligand-binding domain (LBD) of the human Retinoid X Receptor beta (RXR beta) fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer™UAS-bla HEK 293T cell line. GeneBLAzer™UAS-bla HEK 293T cells stably express a beta-lactamase reporter gene under the transcriptional control of an upstream activator sequence (UAS). When an agonist binds to the LBD of the GAL4 (DBD)-RXR beta (LBD) fusion protein, the protein binds to the UAS, resulting in expression of beta-lactamase. RXR beta-UAS-bla HEK 293T cells are functionally validated for Z' and EC₅₀ concentrations of 9-cis retinoic acid (9-cis-RA). In addition, RXR beta-UAS-bla HEK 293T cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, and stimulation time.

Specifications

• Alternate Agonists: Progesterone
• Antagonists: RU 486
• Assay Entry: Cell-based beta lactamase reporter gene
• Cell State: Dividing Cells
• Content And Storage: RXR beta-UAS-bla HEK 293T cells (K1259)
  
  Shipping: Dry Ice
  Storage: -80°C (liquid nitrogen)
• Detection Method: Fluorescence
• Druggable Target: Nuclear Receptors
• No. of Cells: 2 x 10⁶ Cells
• Primary Agonist: R5020
• Product Line: GeneBLAzer™
• Product Type: HEK 293T Cells
• Quantity: 2 x 10⁶ cells
• Reporter Gene: BLA (Beta-Lactamase)
• Target Entry: RXR beta, NR2B2, RXRB

For Research Use Only. Not for use in diagnostic procedures.