Thermo Scientific™ GeneBLAzer™ VDR-UAS-bla HEK 293T Cells

Description

VDR-UAS-bla HEK 293T cells contain the ligand-binding domain (LBD) of the human Vitamin D receptor (VDR) fused to the DNAbinding domain of GAL4 stably integrated in the GeneBLAzer™UAS-bla HEK 293T cell line.

VDR-UAS-bla HEK 293T cells contain the ligand-binding domain (LBD) of the human Vitamin D receptor (VDR) fused to the DNAbinding domain of GAL4 stably integrated in the GeneBLAzer™UAS-bla HEK 293T cell line. GeneBLAzer™UAS-bla HEK 293T cells stably express a beta-lactamase reporter gene under the transcriptional control of an upstream activator sequence (UAS). When an agonist binds to the LBD of the GAL4 (DBD)-VDR (LBD) fusion protein, the protein binds to the UAS, resulting in expression of beta-lactamase. Division Arrested (DA) cells are available in two configurations- an Assay Kit (which includes cells and sufficient substrate to analyze 1 x 384-well plate), and a tube of cells sufficient to analyze 10 x 384-well plates. VDR-UAS-bla HEK 293T cells are functionally validated for Z' and EC₅₀ concentrations of Calcitriol (1α, 25-dihydroxyvitamin D3). In addition, VDR-UAS-bla HEK 293T cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, stimulation time, and substrate loading time.

Specifications

• Assay Entry: Cell-based beta lactamase reporter gene
• Cell State: Dividing Cells
• Content And Storage: VDR-UAS-bla HEK 293T cells (K1099)
  
  Shipping: Dry Ice
  Storage: -80°C (liquid nitrogen)
• Detection Method: Fluorescence
• Druggable Target: Nuclear Receptors
• No. of Cells: 2 x 10⁶ Cells
• Primary Agonist: TO901317
• Product Line: GeneBLAzer™
• Product Type: HEK 293T Cells
• Quantity: 2 x 10⁶ cells
• Reporter Gene: BLA (Beta-Lactamase)
• Target Entry: VDR, NR1I1

For Research Use Only. Not for use in diagnostic procedures.