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Thermo Scientific™ GeneBLAzer™ VDR-UAS-bla HEK 293T Cells

Catalog No. K1700
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2 x 106 cells
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K1700 2 x 106 cells
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Catalog No. K1700 Supplier Thermo Scientific™ Supplier No. K1700
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Description

VDR-UAS-bla HEK 293T cells contain the ligand-binding domain (LBD) of the human Vitamin D receptor (VDR) fused to the DNAbinding domain of GAL4 stably integrated in the GeneBLAzer™UAS-bla HEK 293T cell line.

VDR-UAS-bla HEK 293T cells contain the ligand-binding domain (LBD) of the human Vitamin D receptor (VDR) fused to the DNAbinding domain of GAL4 stably integrated in the GeneBLAzer™UAS-bla HEK 293T cell line. GeneBLAzer™UAS-bla HEK 293T cells stably express a beta-lactamase reporter gene under the transcriptional control of an upstream activator sequence (UAS). When an agonist binds to the LBD of the GAL4 (DBD)-VDR (LBD) fusion protein, the protein binds to the UAS, resulting in expression of beta-lactamase. Division Arrested (DA) cells are available in two configurations- an Assay Kit (which includes cells and sufficient substrate to analyze 1 x 384-well plate), and a tube of cells sufficient to analyze 10 x 384-well plates. VDR-UAS-bla HEK 293T cells are functionally validated for Z' and EC50 concentrations of Calcitriol (1α, 25-dihydroxyvitamin D3). In addition, VDR-UAS-bla HEK 293T cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, stimulation time, and substrate loading time.

TRUSTED_SUSTAINABILITY

Specifications

Assay Entry Cell-based beta lactamase reporter gene
Cell State Dividing Cells
Content And Storage VDR-UAS-bla HEK 293T cells (K1099)

Shipping: Dry Ice
Storage: -80°C (liquid nitrogen)
Detection Method Fluorescence
Druggable Target Nuclear Receptors
No. of Cells 2 x 106 Cells
Primary Agonist TO901317
Product Line GeneBLAzer
Product Type HEK 293T Cells
Quantity 2 x 106 cells
Reporter Gene BLA (Beta-Lactamase)
Target Entry VDR, NR1I1
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For Research Use Only. Not for use in diagnostic procedures.

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