Gentamicin Sulfate, MP Biomedicals™

Description

Inhibits the growth of a wide variety of Gram-positive and -negative microorganisms. Gentamicin Sulfate, MP Biomedicals is a broad-spectrum aminoglycoside antibiotic complex. It inhibits bacterial protein biosynthesis by binding to the 30S subunit of the

Gentamicin Sulfate is produced by fermentation of Micromonospora purpurea or M. echinospora. It is a mixture of 3 major components designated as C1, C1a, and C2. Each component consists of five basic nitrogens and requires five equivalents of sulfuric acid per mole of gentamicin base. Gentamicin is used as the sulfate salt.

Gentamicin sulfate is a broad spectrum antibiotic effective against Gram-positive and Gram-negative bacteria. It is effective against strains that are resistant to tetracycline, chloramphenicol, kanamycin and colistin, particularly strains of Pseudomonas, Proteus, Staphylococcus, and Streptococcus.

Mode of action:

• Gentamicin causes codon misreading by binding to the 30S ribosomal subunit
• This binding blocks the translocation of peptidyl-tRNA from the acceptor site to the donor site
• The bactericidal effect on Pseudomonas aeruginosa is exerted by the binding of gentamicin to the outer membrane, where it displaces natural cations, destabilizes the membrane, and forms holes in the cell surface.

Specifications

Chemical Identifiers

• CAS: 1405-41-0
• Synonym: Garamycin, Genoptic, GM Sulfate;Gentiomycin C

• Physical Form: Powder
• pH: 3.5 to 5.5 (4% solution aq. soln.) (literature)
• Melting Point: 218°C to 237°C (literature)
• Potency: >590μg/mg
• Quantity: 50 mg
• Optical Rotation: 116°
• Solubility Information: Soluble in water (50mg/mL, clear to very slightly hazy, colorless to faint). Practically insoluble in alcohol and other organic solvents.
• Grade: Cell Culture

Safety and Handling

Recommended Storage

Store at 4°C.