GFAP Antibody (GA-5) - Azide and BSA Free, Novus Biologicals™

Mouse Monoclonal Antibody has been used in 2 publications

Supplier:  Novus Biologicals NBP233184

Catalog No. NBP233184

Description

GFAP Monoclonal specifically detects GFAP in Human, Mouse, Rat, Porcine, Bovine, Chicken, Rabbit samples. It is validated for Western Blot, Simple Western, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen.

Specifications

• Antigen: GFAP
• Classification: Monoclonal
• Concentration: 1.0 mg/mL
• Dilution: Western Blot, Simple Western, Flow Cytometry, ELISA 1-5ug/ml for coating, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen
• Gene Symbols: GFAP
• Immunogen: GFAP isolated from pig spinal cord (Uniprot: P14136)
• Purification Method: Protein G purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: This monoclonal antibody recognizes a protein of ∼50kDa which is identified as Glial Fibrillary Acidic Protein (GFAP). It shows no cross-reaction with other intermediate filament proteins. GFAP is specifically found in astroglia. GFAP is a very popular marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system. Antibody to GFAP is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS.
• Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Isotype: IgG1 κ

• Applications: Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen)
• Clone: GA-5
• Conjugate: Unconjugated
• Gene Alias: FLJ45472, GFAP astrocytes, glial fibrillary acidic protein
• Host Species: Mouse
• Molecular Weight of Antigen: 50 kDa
• Quantity: 0.1 mg
• Research Discipline: Astrocyte Markers, Cancer, Cell Biology, Cellular Markers, Chaperone Mediated Autophagy (CMA), Inflammation, Neurodegeneration, Neuroscience, Signal Transduction, Stem Cell Markers
• Gene ID (Entrez): 2670
• Target Species: Human, Mouse, Rat, Pig, Bovine, Chicken, Rabbit
• Form: Purified

For Research Use Only