GFAP Monoclonal Antibody (GA5), eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Supplier:  LIFE TECHNOLOGIES 14989237

Catalog No. 14-989-237

Description

This GA5 MAb reacts with human, mouse, rat, chicken, rabbit, and pig glial fibrillary acidic protein (GFAP). This 49-kDa type III intermediate filament protein is expressed in neural tissues and distinguishes astrocytes from other glial cells during central nervous system development. Three alternative splice variants of GFAP exist; however, alpha-GFAP is the predominant form expressed in astrocytes. GFAP can co-assemble with vimentin and nestin in astrocytes, but such associations are not required for assembly. Like other intermediate filaments, GFAP assembly is dependent on phosphorylation and dephosphorylation of the N-terminal domain. Studies have demonstrated that mutations in the GFAP gene lead to Alexander disease. Moreover, GFAP has also been shown to be overexpressed in certain glial-derived tumors. Applications Reported: This GA5 antibody has been reported for use in immunocytochemistry, western blotting, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, and flow cytometric analysis. Applications Tested: This GA5 antibody has been tested by immunoblotting of lysate prepared from mouse brain, immunocytochemistry of fixed and permeabilized C6 cells, and immunohistochemistry of FFPE human tissue using low pH antigen retrieval. This can be used at less than or equal to 5 μg/mL. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 μm post-manufacturing filtered.

GFAP Monoclonal antibody specifically detects GFAP in Chicken, Human, Mouse, Porcine, Rabbit, Rat samples. It is validated for Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Western Blot

Specifications

• Antigen: GFAP
• Classification: Monoclonal
• Concentration: 0.5 mg/mL
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Accession No.: P03995, P14136, P47819
• Host Species: Mouse
• Quantity: 2 mg
• Primary or Secondary: Primary
• Target Species: Chicken, Human, Mouse, Porcine, Rabbit, Rat
• Product Type: Antibody
• Isotype: IgG1

• Applications: Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Western Blot
• Clone: GA5
• Conjugate: Unconjugated
• Gene: GFAP
• Gene Alias: AI836096; ALXDRD; cb345; class III intermediate filament protein; etID36982.3; FLJ45472; gfap; GFAP epsilon; gfap protein; gfapl; Glial fibrillary acidic protein; glial fibrillary acidic protein alpha; I79_011607; intermediate filament; intermediate filament protein; intermediate filament protein; GFAP; Unknown (protein for MGC:139638); wu:fb34h11; wu:fk42c12; xx:af506734; zgc:110485; zGFAP; intermediate filament protein; zrf-1 antigen
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 100352532, 14580, 24387, 2670, 396562, 419969
• Content And Storage: 4° C
• Form: Liquid