GFP Antibody, R&D Systems™

Mouse Monoclonal Antibody

Supplier:  R&D Systems MAB42401SP

Catalog No. MAB42401SP

Description

GFP Monoclonal specifically detects GFP in Multi-Species samples. It is validated for Immunoprecipitation, Intracellular Staining by Flow Cytometry, Immunocytochemistry, CyTOF-ready.

Specifications

• Antigen: GFP
• Classification: Monoclonal
• Concentration: LYOPH
• Dilution: Immunoprecipitation 2 ug/100 ug cell lysate, Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells, Immunocytochemistry 8-25 ug/mL, CyTOF-ready
• Gene Accession No.: P42212
• Gene Symbols: GFP
• Immunogen: E. coli-derived recombinant GFPuv Ser2-Lys238 Accession # P42212
• Quantity: 25 μg
• Primary or Secondary: Primary
• Reconstitution: Reconstitute at 0.5 mg/mL in sterile PBS.
• Content And Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Isotype: IgG1

• Applications: Immunoprecipitation, Flow Cytometry, Immunocytochemistry, CyTOF
• Clone: 454505
• Conjugate: Unconjugated
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS. with No Preservative
• Gene Alias: eGFP, Enhanced Green Fluorescent Protein, GFPuv, Green Fluorescent Protein, green fluorescent protein (gfp)
• Host Species: Mouse
• Purification Method: Protein A or G purified from hybridoma culture supernatant
• Regulatory Status: RUO
• Test Specificity: Stains eGFP transfectants but not irrelevant transfectants in flow cytometry. Detects recombinant GFPuv in Western blots. For optimal Western blot results, we recommend R&D Systems Goat Anti-GFP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4240 ) or Novus Biologicals Mouse Anti-GFP Monoclonal Antibody (Catalog # NBP2-2211 ).
• Target Species: Multi-species
• Form: Purified

For Research Use Only