Invitrogen™ GFP Monoclonal Antibody (1F1)

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
PIMA547290	Invitrogen™ MA547290	500 μL
PIMA547387	Invitrogen™ MA547387	100 μL

Description

GFP Monoclonal Antibody for Western Blot, ICC/IF, IHC

The jellyfish Aequorea victoria contains green fluorescent protein (GFP Tag) that emits light in the bioluminescence reaction of the animal. GFP has been used widely as a reporter protein for gene expression in eukaryotic and prokaryotic organisms, and as a protein tag in cell culture and in multicellular organisms. As a fusion tag, GFP can be used to localize proteins, to study their movement or to research the dynamics of the subcellular compartments where these proteins are targeted. GFP technology has revealed considerable new insights in the physiological activities of living cells. GFP is a 27 kDa monomeric protein, which autocatalytically forms a fluorescent pigment. The wild type protein absorbs blue light (maximally at 395nm) and emits green light (peak emission 508nm) in the absence of additional proteins, substrates, or co-factors. GFP fluorescence is stable, species independent and is suitable for a variety of applications. GFP has been used extensively as a fluorescent tag to monitor gene expressin and protein localization. Moreover, other applications for GFP include its use in assessing protein protein interactions in the yeast two hybrid system, and in measuring distances between proteins in fluorescence energy transfer (FRET) experiments.

Specifications

• Antigen: GFP
• Concentration: 1 mg/mL
• Applications: Immunohistochemistry, Western Blot, Immunocytochemistry
• Conjugate: Unconjugated
• Host Species: Mouse
• Regulatory Status: RUO
• Formulation: PBS with 50% glycerol and 5mM sodium azide
• Immunogen: Recombinant protein purified from E. coli. The epitope is in the N-terminal 18 amino acids of the protein, the peptide MVSKGAELFTGIVPILIE, which is found in the Clontech and other GFP vectors.
• Product Type: Antibody

• Clone: 1F1
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Classification: Monoclonal
• Form: Liquid
• Isotype: IgM
• Target Species: Tag
• Gene Alias: GFP; GFP tag; GFP2; green fluorescence; green fluorescent; Turbo eGFP
• Primary or Secondary: Primary
• Purification Method: Protein A/G