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Invitrogen™ GFP Monoclonal Antibody (4B10B2)
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Catalog No. PIMA515349
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100 μg
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PIMA515349 100 μg
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Catalog No. PIMA515349 Supplier Invitrogen™ Supplier No. MA515349
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Description

Mouse Monoclonal Antibody

MA5-15349 targets GFP in indirect ELISA, flow cytometry, immunohistochemistry, and Western blot applications and shows reactivity with Tag samples. The MA5-15349 immunogen is purified recombinant fragment of GFP expressed in E. Coli. MA5-15349 detects GFP which has a predicted molecular weight of approximately 27kDa.

The jellyfish Aequorea victoria contains green fluorescent protein (GFP Tag) that emits light in the bioluminescence reaction of the animal. GFP has been used widely as a reporter protein for gene expression in eukaryotic and prokaryotic organisms, and as a protein tag in cell culture and in multicellular organisms. As a fusion tag, GFP can be used to localize proteins, to study their movement or to research the dynamics of the subcellular compartments where these proteins are targeted. GFP technology has revealed considerable new insights in the physiological activities of living cells. GFP is a 27 kDa monomeric protein, which autocatalytically forms a fluorescent pigment. The wild type protein absorbs blue light (maximally at 395nm) and emits green light (peak emission 508nm) in the absence of additional proteins, substrates, or co-factors. GFP fluorescence is stable, species independent and is suitable for a variety of applications. GFP has been used extensively as a fluorescent tag to monitor gene expressin and protein localization. Moreover, other applications for GFP include its use in assessing protein protein interactions in the yeast two hybrid system, and in measuring distances between proteins in fluorescence energy transfer (FRET) experiments.
TRUSTED_SUSTAINABILITY

Specifications

Antigen GFP
Applications ELISA, Flow Cytometry, Immunohistochemistry (Paraffin), Western Blot
Classification Monoclonal
Clone 4B10B2
Concentration 1.0 mg/mL
Conjugate Unconjugated
Formulation PBS with 0.03% sodium azide
Gene Alias GFP; GFP tag; GFP2; green fluorescence; green fluorescent; Turbo eGFP
Host Species Mouse
Immunogen Purified recombinant fragment of GFP expressed in E. Coli.
Purification Method Protein G
Quantity 100 μg
Regulatory Status RUO
Primary or Secondary Primary
Target Species Tag
Content And Storage Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Product Type Antibody
Form Liquid
Isotype IgG2a
Target Molecule Tag
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