Invitrogen™ GFP Monoclonal Antibody (GF28R), DyLight™ 650

Catalog No.	Quantity
MA515256D65	50 μL

Catalog No. MA515256D65 Supplier Invitrogen™ Supplier No. MA515256D650

Description

Mouse Monoclonal Antibody

MA5-15256-D650 has been successfully used for immunofluorescence. DyLight 650 has an excitation/emission of 652/672 nm.

The jellyfish Aequorea victoria contains green fluorescent protein (GFP Tag) that emits light in the bioluminescence reaction of the animal. GFP has been used widely as a reporter protein for gene expression in eukaryotic and prokaryotic organisms, and as a protein tag in cell culture and in multicellular organisms. As a fusion tag, GFP can be used to localize proteins, to study their movement or to research the dynamics of the subcellular compartments where these proteins are targeted. GFP technology has revealed considerable new insights in the physiological activities of living cells. GFP is a 27 kDa monomeric protein, which autocatalytically forms a fluorescent pigment. The wild type protein absorbs blue light (maximally at 395nm) and emits green light (peak emission 508nm) in the absence of additional proteins, substrates, or co-factors. GFP fluorescence is stable, species independent and is suitable for a variety of applications. GFP has been used extensively as a fluorescent tag to monitor gene expressin and protein localization. Moreover, other applications for GFP include its use in assessing protein protein interactions in the yeast two hybrid system, and in measuring distances between proteins in fluorescence energy transfer (FRET) experiments.

Specifications

• Antigen: GFP
• Applications: Immunocytochemistry
• Classification: Monoclonal
• Clone: GF28R
• Concentration: 1 mg/mL
• Conjugate: DyLight 650
• Formulation: PBS with proprietary stabilizer and 0.02% sodium azide
• Gene Alias: GFP; GFP tag; GFP2; green fluorescence; green fluorescent; green fluorescent protein; Turbo eGFP
• Host Species: Mouse
• Immunogen: GFP from the jellyfish Aequorea Victoria N-terminal peptide-KLH conjugated.
• Purification Method: Protein A
• Quantity: 50 μL
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Tag
• Content And Storage: 4°C, do not freeze
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG1
• Target Molecule: Tag