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GFP, Mouse anti-All, Clone: 2B6, Millipore Sigma™

Catalog No. MABC168925 Shop All MilliporeSigma Products
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MABC168925 25 μg
MABC1689100 100 μg
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Catalog No. MABC168925 Supplier MilliporeSigma Supplier No. MABC168925UG
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Description

Mouse Monoclonal Antibody

Anti-GFP, clone 2B6, Cat. No. MABC1689, is a mouse monoclonal antibody that detects GFP-conjugated to most proteins and has been tested for use in Immunocytochemistry and Western Blotting.

Green fluorescent protein (UniProt: P42212) is encoded by the GFP gene in Aequorea victoria (Jellyfish). Green fluorescence protein (GFP) is a 27 kDa protein from jellyfish that serves as an energy-transfer acceptor. It transduces the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer and it fluoresces in vivo upon receiving energy from the Ca2+-activated photoprotein aequorin. GFP has an absorption max at 395 nm and the emission peaks at 507 to 510 nm. However, the exact value of emission max depends on the environment of the chromophore. GFP is an important tool in cell biology research that displays stability under fixation conditions and is suitable for a variety of applications, such as tracing cell lineage tracer, reporter of gene expression, or exploring protein-protein interactions.
TRUSTED_SUSTAINABILITY

Specifications

Antigen GFP
Applications Immunocytochemistry, Western Blot
Classification Monoclonal
Clone 2B6
Conjugate Unconjugated
Dilution Immunocytochemistry Analysis: A 1:50 dilution from a representative lot detected GFP in HEK293T cells stabely transfected with GFP-tagged Aquaporin 4.
Formulation Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Gene Alias Green fluorescent protein
Gene Symbols GFP
Host Species Mouse
Immunogen His-tagged recombinant jellyfish Green fluorescent protein.
Purification Method Protein G purified
Quantity 25 μg
Regulatory Status RUO
Research Discipline Secondary & Control Antibodies
Primary or Secondary Primary
Test Specificity Clone 2B6 is a mouse monoclonal antibody that detects GFP-conjugated to most proteins.
Target Species All species
Content And Storage Stable for 1 year at 2-8°C from date of receipt.
Form Purified
Isotype IgG1 κ
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