GFP Mouse anti-Tag, AP, Clone: 9F9.F9, Rockland™

Catalog No.	Quantity
50-199-1651	1 mg

Catalog No. 50-199-1651 Supplier Rockland Immunochemicals Supplier No. 600305215

Description

Mouse Monoclonal Antibody

Store vial at 4°C before opening. DO NOT FREEZE. This product is stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse Serum, anti-Alkaline Phosphatase, purified and partially purified Green Fluorescent Protein (Aequorea victoria). Reactivity is observed against recombinant Green Fluorescent Protein (000-001-215, recombinant GFP from Aequorea victoria) by both Western blot and ELISA. No reaction is seen against RFP.

The jellyfish Aequorea victoria contains green fluorescent protein (GFP Tag) that emits light in the bioluminescence reaction of the animal. GFP has been used widely as a reporter protein for gene expression in eukaryotic and prokaryotic organisms, and as a protein tag in cell culture and in multicellular organisms. As a fusion tag, GFP can be used to localize proteins, to study their movement or to research the dynamics of the subcellular compartments where these proteins are targeted. GFP technology has revealed considerable new insights in the physiological activities of living cells. GFP is a 27 kDa monomeric protein, which autocatalytically forms a fluorescent pigment. The wild type protein absorbs blue light (maximally at 395nm) and emits green light (peak emission 508nm) in the absence of additional proteins, substrates, or co-factors. GFP fluorescence is stable, species independent and is suitable for a variety of applications. GFP has been used extensively as a fluorescent tag to monitor gene expressin and protein localization. Moreover, other applications for GFP include its use in assessing protein protein interactions in the yeast two hybrid system, and in measuring distances between proteins in fluorescence energy transfer (FRET) experiments.

Specifications

• Antigen: GFP
• Applications: ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot
• Classification: Monoclonal
• Clone: 9F9.F9
• Concentration: 1 mg/mL
• Conjugate: Alkaline Phosphatase (AP)
• Formulation: 0.05M tris HCl with 0.0001M ZnCl2, 0.001M MgCl2, 0.15M NaCl, 10 mg/mL BSA, 50% glycerol and 0.01% sodium azide; pH 8
• Gene Alias: GFP; GFP tag; GFP2; Turbo eGFP
• Host Species: Mouse
• Quantity: 1 mg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Content And Storage: 4°C, do not freeze
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG1 κ
• Target Molecule: Tag