Invitrogen™ GFP Polyclonal Antibody

Rabbit Polyclonal Antibody

Catalog Number	Mfr. No.	Quantity
PIPA5143510	Invitrogen™ PA5143510	500 μL
PIPA5143608	Invitrogen™ PA5143608	100 μL

Description

GFP Polyclonal Antibody for Western Blot, ICC/IF

The jellyfish Aequorea victoria contains green fluorescent protein (GFP Tag) that emits light in the bioluminescence reaction of the animal. GFP has been used widely as a reporter protein for gene expression in eukaryotic and prokaryotic organisms, and as a protein tag in cell culture and in multicellular organisms. As a fusion tag, GFP can be used to localize proteins, to study their movement or to research the dynamics of the subcellular compartments where these proteins are targeted. GFP technology has revealed considerable new insights in the physiological activities of living cells. GFP is a 27 kDa monomeric protein, which autocatalytically forms a fluorescent pigment. The wild type protein absorbs blue light (maximally at 395nm) and emits green light (peak emission 508nm) in the absence of additional proteins, substrates, or co-factors. GFP fluorescence is stable, species independent and is suitable for a variety of applications. GFP has been used extensively as a fluorescent tag to monitor gene expressin and protein localization. Moreover, other applications for GFP include its use in assessing protein protein interactions in the yeast two hybrid system, and in measuring distances between proteins in fluorescence energy transfer (FRET) experiments.

Specifications

• Antigen: GFP
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Classification: Polyclonal
• Form: Liquid
• Isotype: IgG
• Target Species: Tag
• Gene Alias: GFP; GFP tag; GFP2; green fluorescence; green fluorescent; Turbo eGFP
• Primary or Secondary: Primary
• Purification Method: Affinity chromatography

• Concentration: 1 mg/mL
• Applications: Western Blot, Immunocytochemistry
• Conjugate: Unconjugated
• Host Species: Rabbit
• Regulatory Status: RUO
• Formulation: PBS with 50% glycerol and 5mM sodium azide
• Immunogen: Recombinant AcGFP expressed in and purified from E. coli.
• Product Type: Antibody