Invitrogen™ GFP Recombinant Rabbit Monoclonal Antibody

Catalog No.	Quantity
G10362	100 μg

Catalog No. G10362 Supplier Invitrogen™ Supplier No. G10362

Description

Rabbit Recombinant Monoclonal Antibody

Storage and reconstitution: to prepare a 0.2 mg/mL stock solution, reconstitute the lyophilized antibody in 0.5mL PBS, pH 7.4. Reconstituted product can be stored for up to 3months at 4°C with the addition of 2mM sodium azide. Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

The jellyfish Aequorea victoria contains green fluorescent protein (GFP Tag) that emits light in the bioluminescence reaction of the animal. GFP has been used widely as a reporter protein for gene expression in eukaryotic and prokaryotic organisms, and as a protein tag in cell culture and in multicellular organisms. As a fusion tag, GFP can be used to localize proteins, to study their movement or to research the dynamics of the subcellular compartments where these proteins are targeted. GFP technology has revealed considerable new insights in the physiological activities of living cells. GFP is a 27 kDa monomeric protein, which autocatalytically forms a fluorescent pigment. The wild type protein absorbs blue light (maximally at 395nm) and emits green light (peak emission 508nm) in the absence of additional proteins, substrates, or co-factors. GFP fluorescence is stable, species independent and is suitable for a variety of applications. GFP has been used extensively as a fluorescent tag to monitor gene expressin and protein localization. Moreover, other applications for GFP include its use in assessing protein protein interactions in the yeast two hybrid system, and in measuring distances between proteins in fluorescence energy transfer (FRET) experiments.

Specifications

• Antigen: GFP
• Applications: ELISA, Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Western Blot, Immunocytochemistry
• Classification: Recombinant Monoclonal
• Concentration: 0.2 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 0.09% sodium azide
• Gene Alias: GFP; GFP tag; GFP2; green fluorescence; green fluorescent; Turbo eGFP
• Host Species: Rabbit
• Immunogen: Full-length GFP.
• Purification Method: Protein A
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Tag
• Content And Storage: -20°C, store in dark
• Product Type: Antibody
• Form: Lyophilized
• Isotype: IgG
• Target Molecule: Tag