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Edvotek™ GFP Transformation Extension: Colony PCR Experiment Kit
Determines whether cloning, transformation experiments were successful.
Supplier: Edvotek™ 323/DEL
Includes: Instructions, PCR EdvoBeads™, Colony Primer Mix Concentrate, Control DNA Concentrate, Ampicillin, IPTG, EdvoQuick™ DNA Ladder, TE Buffer, BactoBeads™, ReadyPour™ Luria Broth Agar, Petri Dishes, , ampicillin, IPTG, ReadyPour™ Luria Broth Agar (sterile), UltraSpec-Agarose™, Electrophoresis Buffer (50X), 10X Gel Loading Solution, InstaStain™ Ethidium Bromide, FlashBlue™ Liquid Stain, Petri Plates, Sterile Loops, Microcentrifuge & PCR Tubes & Wax Beads.
Description
- Colony PCR to analyze bacteria transformed with pFluoroGreen
- Single colony used as DNA template for PCR
- Resulting PCR sample analyzed using agarose gel electrophoresis
- Used in conjunction Edvotek Transformation Kits (Part No: 222, 223 or 303)
Specifications
GFP transformation, colony PCR Kit | |
Instructions, control BactoBeads™, ampicillin, IPTG, ReadyPour™ Luria Broth Agar (sterile), Petri Plates, sterile loops, PCR EdvoBeads™, control DNA and primers, microcentrifuge tubes, PCR tubes, agarose, DNA ladder, practice gel loading | |
Refrigeration required |
Educational purpose | |
30 minutes (set up), 2 hours or overnight (PCR), 45 minutes (electrophoresis) | |
10 Lab Groups |
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