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Edvotek™ GFP Transformation Extension: Colony PCR Experiment Kit
SCI_ED

Determines whether cloning, transformation experiments were successful.

Supplier:  Edvotek™ 323/DEL

Catalog No. S42874


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Includes: Instructions, PCR EdvoBeads™, Colony Primer Mix Concentrate, Control DNA Concentrate, Ampicillin, IPTG, EdvoQuick™ DNA Ladder, TE Buffer, BactoBeads™, ReadyPour™ Luria Broth Agar, Petri Dishes, , ampicillin, IPTG, ReadyPour™ Luria Broth Agar (sterile), UltraSpec-Agarose™, Electrophoresis Buffer (50X), 10X Gel Loading Solution, InstaStain™ Ethidium Bromide, FlashBlue™ Liquid Stain, Petri Plates, Sterile Loops, Microcentrifuge & PCR Tubes & Wax Beads.

Description

Description

  • Colony PCR to analyze bacteria transformed with pFluoroGreen
  • Single colony used as DNA template for PCR
  • Resulting PCR sample analyzed using agarose gel electrophoresis
  • Used in conjunction Edvotek Transformation Kits (Part No: 222, 223 or 303)
Specifications

Specifications

GFP transformation, colony PCR Kit
Instructions, control BactoBeads™, ampicillin, IPTG, ReadyPour™ Luria Broth Agar (sterile), Petri Plates, sterile loops, PCR EdvoBeads™, control DNA and primers, microcentrifuge tubes, PCR tubes, agarose, DNA ladder, practice gel loading
Refrigeration required
Educational purpose
30 minutes (set up), 2 hours or overnight (PCR), 45 minutes (electrophoresis)
10 Lab Groups
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