Edvotek™ GFP Transformation Extension: Colony PCR Experiment Kit

Description

Includes

Instructions, PCR EdvoBeads™, Colony Primer Mix Concentrate, Control DNA Concentrate, Ampicillin, IPTG, EdvoQuick™ DNA Ladder, TE Buffer, BactoBeads™, ReadyPour™ Luria Broth Agar, Petri Dishes, , ampicillin, IPTG, ReadyPour™ Luria Broth Agar (sterile), UltraSpec-Agarose™, Electrophoresis Buffer (50X), 10X Gel Loading Solution, InstaStain™ Ethidium Bromide, FlashBlue™ Liquid Stain, Petri Plates, Sterile Loops, Microcentrifuge & PCR Tubes & Wax Beads.

Determines whether cloning, transformation experiments were successful.

• Colony PCR to analyze bacteria transformed with pFluoroGreen
• Single colony used as DNA template for PCR
• Resulting PCR sample analyzed using agarose gel electrophoresis
• Used in conjunction Edvotek Transformation Kits (Part No: 222, 223 or 303)

Specifications

• Product Type: PCR Kit
• Grade: 9 to Undergraduate
• For Use With (Application): Educational purpose
• Includes: Instructions, control BactoBeads™, ampicillin, IPTG, ReadyPour™ Luria Broth Agar (sterile), Petri Plates, sterile loops, PCR EdvoBeads™, control DNA and primers, microcentrifuge tubes, PCR tubes, agarose, DNA ladder, practice gel loading
• Time Range: 30 minutes (set up), 2 hours or overnight (PCR), 45 minutes (electrophoresis)
• Content And Storage: Refrigeration required
• Class Size: 10 Lab Groups