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Gibco™ Ampicillin, sodium salt, irradiated
Description
Ampicillin differs from penicillin only by the presence of an amino group, which facilitates penetration through the outer membrane of some gram-negative bacteria. Ampicillin acts by interfering directly with the turnover of the bacteria cell wall and indirectly by triggering the release of enzymes that further alter the cell wall.
- Used as selective antibiotic for resistant bacteria, generally at concentration of 10-25μg/ml for liquid media and 35-50μg/ml for plates
- Product is provided as powder
- Made into stock solution at 10mg/ml in water
- Ampicillin is not used to prevent bacterial contamination of cell cultures due to concerns of mammalian and insect cell toxicity
Cloning, Transformation
Order Info
Shipping Condition: Room Temperature
Compliance
Manufactured at a cGMP compliant facility in Grand Island, NY. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.
Specifications
Specifications
| Content And Storage | Storage conditions: 2 to 8°C Shipping conditions: Ambient Shelf life: 24 months from date of manufacture |
| Concentration | 20 to 125 μg/mL |
| Form | Powder |
| Product Type | Antibiotic |
| For Use With (Application) | Bacterial Selection |
| Quantity | 200 mg |
| Shipping Condition | Room Temperature |
Frequently Asked Questions (FAQs)
For best results, optimal concentrations for selection should be determined empirically in each unique experiment through dose response curves. However, to get a general idea of concentrations that have worked for individual cell types, please click on the following url: http://www.thermofisher.com/us/en/home/life-science/cell-culture/transfection/selection.html or type in “Selection Antibiotics” into our main search on www.thermofisher.com.
No. B-lactamase is targeted to specific linkages in the bacterial cell wall. Since eukaryotic cells lack a cell wall, ampicillin has no effect upon eukaryotic cells.
When an irreplaceable culture becomes contaminated, researchers may attempt to eliminate or control the contamination.
1. Determine if the contamination is bacteria, fungus, mycoplasma, or yeast. Read more here to view characteristics of each contaminant.
2. Isolate the contaminated culture from other cell lines.
3. Clean incubators and laminar flow hoods with a laboratory disinfectant, and check HEPA filters.
4. Antibiotics and antimycotics at high concentrations can be toxic to some cell lines. Therefore, perform a dose-response test to determine the level at which an antibiotic or antimycotic becomes toxic. This is particularly important when using an antimycotic such as Gibco Fungizone reagent or an antibiotic such as tylosin.
The following is a suggested procedure for determining toxicity levels and decontaminating cultures:
1. Dissociate, count, and dilute the cells in antibiotic-free media. Dilute the cells to the concentration used for regular cell passage.
2. Dispense the cell suspension into a multiwell culture plate or several small flasks. Add the antibiotic of choice to each well in a range of concentrations. For example, we suggest the following concentrations for Gibco Fungizone reagent: 0.25, 0.50, 1.0, 2.0, 4.0, and 8.0 µg/mL.
3. Observe the cells daily for signs of toxicity such as sloughing, appearance of vacuoles, decrease in confluency, and rounding.
4. When the toxic antibiotic level has been determined, culture the cells for two to three passages using the antibiotic at a concentration one- to two-fold lower than the toxic concentration.
5. Culture the cells for one passage in antibiotic-free media.
6. Repeat step 4.
7. Culture the cells in antibiotic-free medium for four to six passages to determine if the contamination has been eliminated.
Please view the following page to browse the cell culture antibiotics we offer (https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/antibiotics.html).
Safety and Handling
For Research Use Only. Not for use in diagnostic procedures.
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