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Gibco™ Blasticidin S HCl, powder
Description
Blasticidin S is a peptidyl nucleoside antibiotic isolated from Streptomyces griseochromogenes. It is a potent inhibitor of protein synthesis in bacteria and eukaryotes, while also active in fungi, nematodes, and tumor cells. Blasticidin S acts by blocking hydrolysis of peptidyl-tRNA induced by release factors and inhibits peptide bond formation. It is used as a selection agent in both mammalian and bacterial cells. The recommended working concentration ranges from 1 to 30 μg/mL depending on the cell line and 25–100 μg/mL for bacterial selection. Cell death occurs rapidly, and blasticidin-resistant stable mammalian cell lines can be generated in less than one week.
- Blasticidin S acts quickly by inhibiting peptide bond formation by the ribosome
- Used for prevention of contamination of cell cultures
- Recommended working concentration ranges from 2 to 2μg/mL depending on the cell line
- Resistance is conferred by expression of either one of two Blasticidin S deaminase genes: BSD (2) or bsr
Resistance to blasticidin S is conferred by BSR and BSD, isolated from Bacillus cereus K55-S and Aspergillus terreus respectively. The BSR resistance gene encodes blasticidin S deaminase, which catalyzes the conversion of blasticidin S to deaminohydroxyblasticidin S. Deaminohydroxyblasticidin S is a biologically inactive derivative of blasticidin S and does not interact with or inhibit prokaryotic or eukaryotic ribosomes. The BSD resistance gene also encodes a blasticidin S deaminase, which catalyzes a similar reaction to the BSR deaminase.
For bacterial selection purposes, the salt content of the LB medium must remain low (<90 mM) and the pH should not exceed 7.0 to maintain the activity of basticidin S. A kill curve is recommended in order to determine the minimum effective blasticidin S concentration to kill non-resistant cells.
Applications: Cell Culture, Cloning, Drosophila S2 Cell Culture, High Five™ Cell Culture, Insect Cell Culture, Mammalian Cell Culture, Selection, Sf9 & Sf21 Cell Culture, Transfection, Transformation
Order Info
Shipping conditions: Ambient
Specifications
Specifications
| Color | White, off-white |
| Content And Storage | Storage conditions: -5 to -30°C Shipping conditions: room temperature Shelf life: 48 months from date of manufacture |
| Cell Type | Eukaryotic Cells, Prokaryotic Cells |
| Concentration | 50 to 100 μg/mL |
| Culture Type | Mammalian Cell Culture, Insect Cell Culture |
| Form | Powder |
| Product Type | Antibiotic |
| Sterility | Sterile |
| With Additives | HEPES |
| For Use With (Application) | Bacterial Selection |
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Safety and Handling
For Research Use Only. Not for use in diagnostic procedures.
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