Gibco™ Collagenase, Type II, powder

Catalog No.	Quantity
17-101-015	1 g

Catalog No. 17-101-015 Supplier Gibco™ Supplier No. 17101015

Description

Cleaves bond between neutral amino acid (X) and glycine in sequence Pro-X-Glyc-Pro, which is found with high frequency in collagen

Collagenase is unique among proteases in its ability to degrade the triple-helical native collagen fibrils commonly found in connective tissues such as skin, tendon, blood vessels, and bone. Collagenase disaggregation is suitable for the culture of human tumors, mouse kidney, human adult and fetal brain, and many other tissues including epithelium. Collagenase is relatively gentle, dissociates well at physiological temperature and pH, and requires no mechanical agitation or special equipment.

• Isolated from Clostridium histolyticum
• Packaged as lyophilized, non-sterile powder for research use in cell or tissue dissociation and organ perfusions
• Activity is >125 units/mg
• With higher clostripain activity and is well-suited for digestion of heart, bone, thyroid, cartilage, and liver tissues

Cell Culture, Mammalian Cell Culture

Order Info

Shipping Condition: Room Temperature

Specifications

• Content And Storage: Storage conditions: 2°C to 8°C. Protect from light.
  Shipping conditions: Room temperature
  Shelf life: 24 months from date of manufacture
• Form: Lyophilized
• Product Type: Collagenase
• Sterility: Non-sterile
• Quantity: 1 g
• Shipping Condition: Room Temperature

Frequently Asked Questions (FAQs)

Can I reconstitute Collagenase, Type II, powder (Cat. No. 17101015) into DMEM or MEM alpha instead of HBSS with Ca and Mg?

Yes, Collagenase, Type II, powder can be reconstituted in DMEM or MEM alpha as long as the Mg and Ca concentrations in the medium are similar.

How do I make the 1000X stock (100 U/µL) solution from Collagenase powder?

1. Add 1 mL Hanks' Balanced Salt Solution (HBSS) with calcium and magnesium directly to 1 g vial of Collagenase. Vortex gently to ensure complete dissolution. Transfer to a clean tube.
2. Determine volume of HBSS (with calcium and magnesium) required to bring collagenase solution to 100 U/µL (1000X stock solution). The activity is lot- specific. Rinse vial with this volume of HBSS (with calcium and magnesium), and combine. Filter sterilize 1000X stock solution with a low protein binding filtration unit.
Example: Assuming the lot you have purchased has an activity of 265 U/mg, this lot will have 265000 Units per mL when you reconstitute collagenase into HBSS (with calcium and magnesium) at 1 g/mL. In order to dilute 265000 U/L to 100000 U/mL (= 100 U/µL), you need to dilute the 1 g/mL enzyme solution 2.65 fold.

What reagents do you offer for cell dissociation, and what are the main differences between them?

Please use this selection chart that compares our cell dissociation reagents (https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/reagents/trypsin.html).

Safety and Handling

WARNING: Cancer and Reproductive Harm - www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.