Contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium
Originally formulated with low glucose (1g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate
Contains no proteins, lipids, or growth factors
Requires supplementation, commonly with 10% Fetal Bovine Serum (FBS)
Uses sodium bicarbonate buffer system (3.7g/L), and therefore requires a 5 to 10% CO2 environment to maintain physiological pH
Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, cell lines such as HeLa, 293, Cos-7, and PC-12
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