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Gibco™ DMEM, high glucose, pyruvate
Used as basal medium for supporting the growth of many different mammalian cells. Gibco™ DMEM, High Glucose, Pyruvate is used in cell culture processing applications.
Supplier: Gibco™ 11995115
Description
- DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells
- Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12
- Store at 2°C to 8°C, protected from light
Modified as follows:
- With: High glucose, L-glutamine, phenol red, sodium pyruvate
- Without: HEPES
Using DMEM
- DMEM is unique as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium
- It was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate
- DMEM contains no proteins, lipids, or growth factors therefore it requires supplementation, commonly with 10% Fetal Bovine Serum (FBS)
- DMEM uses a sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH
Order Info
Shipping Condition: Room Temperature
Specifications
| Storage conditions: 2-8° C. Protect from light Shipping conditions: Ambient Shelf life: 12 months from date of manufacture |
|
| Primary Fibroblasts, Neurons, Glial Cells, HUVECs, Smooth Muscle Cells | |
| 1 X | |
| DMEM (Dulbecco's Modified Eagle Medium) | |
| Sterile-filtered | |
| No HEPES | |
| 4500 mg/L | |
| 5 L |
| HeLa, 293, Cos-7, and PC-12 | |
| Animal Origin-free | |
| Liquid | |
| Standard Serum Supplementation | |
| High Glucose, Glutamine, Phenol Red, Sodium Pyruvate | |
| Antibody and cell culture production, Mammalian cell culture | |
| Gibco™, SynerGy™ | |
| Ambient |
Safety and Handling
ShelfLife : 12 Months From Date of Manufacture
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Product Title
When used as a medical device, Federal law restricts this device to sale by or on the order of a physician. Customers using Gibco DMEM in a manufacturing process, who have a submission with the FDA, may request a letter of authorization from Life Technologies to reference their Type II Drug Master File (DMF). For human ex vivo tissue and cell culture processing applications.