Gibco™ DMEM, no glucose, no glutamine, no phenol red

Description

Dulbecco's Modified Eagle Medium (DMEM) is a widely used basal medium for supporting the growth of many different mammalian cells.

Dulbecco's Modified Eagle Medium (DMEM) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. We offer a variety of Gibco™ DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This DMEM is modified as follows:

Without: Glucose, Sodium Pyruvate, HEPES, L-glutamine, Phenol Red

The complete formulation is available.

DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate.

Product Use

For Research Use Only: Not intended for animal or human diagnostic or therapeutic use.

DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L) and therefore requires a 5-10% CO₂ environment to maintain physiological pH.

Certifications

ISO 13485

Specifications

• Cell Line: HeLa, 293, Cos-7, and PC-12
• Cell Type: Primary Fibroblasts, Neurons, Glial Cells, HUVECs, Smooth Muscle Cells
• Classification: Animal Origin-free
• Concentration: 1 X
• Form: Liquid
• Product Type: DMEM (Dulbecco's Modified Eagle Medium)
• Serum Level: Standard Serum Supplementation
• Sterility: Sterile-filtered
• With Additives: Sodium Bicarbonate
• Without Additives: No Glucose, No Glutamine, No HEPES, No Phenol Red, No Sodium Pyruvate
• Manufacturing Quality: cGMP-compliant under the ISO 13485 standard
• Product Line: Gibco™
• Quantity: 500 mL
• Shipping Condition: Room Temperature
• Content And Storage: Storage conditions: 2-8°C. Protect from light.
  
  Shipping conditions: Ambient
  
  Shelf life: 12 months from date of manufacture