Gibco™ Glasgow's MEM, powder

Description

Requires

Supplementation

Originally developed by Ian McPherson and Michael Stoker as modification of Eagle's medium

Glasgow's Minimum Essential Medium (GMEM) was utilized to study the genetic factors which affected cell competence. Glasgow's MEM was developed for use with kidney cells lines, such as BHK-21. Glasgow's MEM is unique from other media as it contains twice the concentration of amino acids and vitamins than the original Basal Medium Eagle, and is used without serum. Glasgow's MEM was originally formulated with 10% tryptose phosphate broth. This MEM is modified as follows: with L-glutamine, phenol red; without tryptose phosphate broth, sodium bicarbonate, HEPES.

• Contains no proteins, lipids, or growth factors
• Requires supplementation, commonly with 10% tryptose phosphate broth
• Uses sodium bicarbonate buffer system (2.75g/L) and therefore requires 5-10% CO₂ environment to maintain physiological pH
• Powder forms of Gibco cell culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation

Cell Culture, Mammalian Cell Culture

Order Info

Shipping Condition: Room Temperature

Compliance

Gibco Glasgow's MEM is manufactured at a cGMP compliant facility located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.

Specifications

• Form: Powder
• With Additives: High Glucose, Glutamine, Phenol Red
• Without Additives: No HEPES, No Sodium Pyruvate
• Product Line: Gibco™
• Quantity: 50 L
• Shipping Condition: Room Temperature
• Content And Storage: Storage conditions: 2-8°C
  Shipping conditions: Ambient
  Shelf life: 36 months from date of manufacture

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov

Not for human or animal therapeutic use. Uses other than the labeled intended use may be a violation of local law. For in vitro diagnostic use.