Gibco™ Ham's F-12 Nutrient Mix, GlutaMAX™ Supplement

Ham's F-12 Nutrient Mixture (F-12) was designed for serum-free single-cell plating of Chinese Hamster Ovary (CHO) cells.

Catalog Number	Mfr. No.	Quantity
31-765-035	Gibco™ 31765035	500 mL
31-765-092	Gibco™ 31765092	10 x 500 mL

Description

Ham's F-12 Nutrient Mixture (F-12) was designed for serum-free single-cell plating of Chinese Hamster Ovary (CHO) cells. F-12 has since been used for serum-free growth of CHO cultures as well as serum-supplemented growth of other mammalian cells, including chondrocytes and rat prostate epithelial cells. We offer a variety of Gibco™ F-12 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This Ham's F-12 is modified as follows:
With: GlutaMAX™, Phenol Red

The complete formulation is available.

Compared to other basal media, F-12 contains a wider variety of components, including zinc, putrescine, hypoxanthine, and thymidine. Serum-free growth of CHO cells in F-12 has led to a variety of improved formulations. Gibco™ F-12 with GlutaMAX™ supplement minimizes toxic ammonia build-up and improves cell viability and growth in an easy-to-use format.

Gibco™ F-12 contains no proteins or growth factors. Therefore, F-12 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). F-12 uses a sodium bicarbonate buffer system (1.176 g/L) and therefore requires a 5-10% CO₂ environment to maintain physiological pH.

Order Info

Shipping Condition: Room Temperature

Specifications

• Content And Storage: Storage conditions: 2-8° C. Protect from light
  Shipping conditions: Ambient
  Shelf life: 12 months from date of manufacture
• Cell Type: Primary Rat Astrocytes
• Concentration: 1 X
• Product Type: Ham's F-12 Nutrient Mixture
• With Additives: GlutaMAX, Phenol Red, Sodium Pyruvate
• Green Features: Sustainable packaging
• Product Line: Gibco™, GlutaMAX™

• Cell Line: CHO, COS-7, and rat prostate epithelial cells
• Classification: Animal Origin-free
• Form: Liquid
• Sterility: Sterile-filtered
• Without Additives: No HEPES
• Manufacturing Quality: cGMP-compliant under the ISO 13485 standard
• Shipping Condition: Room Temperature

Safety and Handling

ShelfLife : 12 Months From Date of Manufacture

For Research Use Only. Not for use in diagnostic procedures. Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law. For in vitro diagnostic use.