Gibco™ MEM (10X), no glutamine

Description

Requires

Supplementation

Minimum Essential Medium (MEM) is one of the most commonly used of all cell culture media.

Minimum Essential Medium (MEM) is one of the most commonly used of all cell culture media. MEM can be used with a variety of suspension and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts, and primary rat astrocytes. We offer a variety of Gibco™ MEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This MEM is modified as follows:

With: Phenol Red

Without: L-glutamine, HEPES, Sodium Bicarbonate

The complete formulation is available.

Gibco™ MEM, developed by Harry Eagle, was based on his earlier formulation of Basal Medium Eagle (BME). Many other modifications of MEM followed, including Glasgow's MEM, MEM α, DMEM, and Temin's Modification. MEM is available with Earle's salts for use in a CO₂ incubator, or with Hanks' salts for use without CO₂. This product is made with Earle's salts.

Using MEM

MEM contains no proteins, lipids, or growth factors. Therefore, MEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). MEM uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5–10% CO₂ environment to maintain physiological pH. Concentrated forms of Gibco™ cell culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (see protocol for details).

Order Info

Shipping Condition: Room Temperature

Specifications

• Cell Line: HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, and fibroblasts
• Cell Type: Primary Rat Astrocytes
• Classification: Animal Origin-free
• Concentration: 10X
• Form: Liquid
• Product Type: MEM (Minimum Essential Medium)
• Serum Level: Standard Serum Supplementation
• Sterility: Sterile-filtered
• With Additives: Low Glucose, Phenol Red
• Without Additives: No Glutamine, No HEPES, No Sodium Pyruvate, No Sodium Bicarbonate
• Manufacturing Quality: cGMP-compliant under the ISO 13485 standard
• Product Line: Gibco™
• Quantity: 500 mL
• Shipping Condition: Room Temperature
• Content And Storage: Storage conditions: 2-8° C. Protect from light
  Shipping conditions: Ambient
  Shelf life: 12 months from date of manufacture

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov

For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.