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Gibco™ MEM α, nucleosides, no phenol red
Description
Requires
Supplementation
Minimum Essential Medium (MEM) α is widely used for mammalian cell culture as well as selection for transfected DHFR negative cells. MEM α can be used with a variety of suspension and adherent mammalian cells, including keratinocytes, primary rat astrocytes, and human melanoma cells. We offer a variety of Gibco™ MEM α modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
This MEM α is modified as follows:
With: Ribonucleosides, Deoxyribonucleosides, L-glutamine
Without: Phenol Red
The complete formulation is available.
Gibco™ MEM α is a modification of Minimum Essential Medium (MEM) that contains non-essential amino acids, sodium pyruvate, lipoic acid, vitamin B12, biotin, and ascorbic acid. MEM α is available without nucleosides for use as a selection medium for DG44 and other DHFR negative cells. This product is made with Earle's salts.
Product Use
For Research Use Only: Not intended for animal or human diagnostic or therapeutic use.
MEM α contains no proteins, lipids, or growth factors. Therefore, MEM α requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). MEM α uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.
Order Info
Shipping Condition: Room Temperature
Compliance
Gibco MEM α is manufactured at a cGMP compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.
Specifications
Specifications
| Cell Line | HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, and fibroblasts |
| Cell Type | Primary Rat Astrocytes |
| Classification | Animal Origin-free |
| Concentration | 1 X |
| Form | Liquid |
| Product Type | MEM α (Minimum Essential Medium α) |
| Serum Level | Standard Serum Supplementation |
| Sterility | Sterile-filtered |
| Sterilization Method | Sterile-filtered |
| With Additives | Low Glucose, Glutamine, Sodium Pyruvate, Ribonucleosides, Deoxyribonucleosides |
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Safety and Handling
For Research Use Only: Not intended for animal or human diagnostic or therapeutic use.
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