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GL7 Monoclonal Antibody (GL-7 (GL7)), eBioscience™, Invitrogen™
Rat Monoclonal Antibody
$95.65 - $243.65
Specifications
| Antigen | GL7 |
|---|---|
| Clone | GL-7 (GL7) |
| Concentration | 0.5 mg/mL |
| Applications | Flow Cytometry, Immunohistochemistry (Frozen), Immunoprecipitation |
| Classification | Monoclonal |
| Catalog Number | Mfr. No. | Quantity | Price | Quantity & Availability | |||||
|---|---|---|---|---|---|---|---|---|---|
| Catalog Number | Mfr. No. | Quantity | Price | Quantity & Availability | |||||
50-133-14
|
Invitrogen
14590281 |
50 μg |
Each
for $95.65
Save 3% Reg : |
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50-133-15
|
Invitrogen
14590282 |
100 μg |
Each for $143.00
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50-133-16
|
Invitrogen
14590285 |
500 μg |
Each
for $243.65
Save 33% Reg : |
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Description
Description: This GL7 monoclonal antibody reacts with a cell-surface protein found on T and B lymphocytes activated in vitro, on bone marrow pre-B-II cells, germinal center B cells, and also human B cell lines Ramos and Daudi. There is strain variability with respect to antigen distribution on thymocytes and Con A-activated spleen cells, with expression in BALB/c greater than that in C57BL/6. GL7 is commonly used as a marker for mouse germinal center B cells. The epitope of GL7 has been identified as a sialic acid glycan moiety called Neu5Ac. This moiety is recognized by CD22. Applications Reported: The GL-7 (GL7) antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistochemical staining of frozen tissue sections. Applications Tested: The GL-7 (GL7) antibody has been tested by flow cytometric analysis of Con A-stimulated mouse splenocytes. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 μm post-manufacturing filtered.
GL7 is a cell-surface protein found on T and B lymphocytes activated in vitro, on bone marrow pre-B-II cells, germinal center B cells, and also human B cell lines Ramos and Daudi. There is strain variability with respect to antigen distribution on thymocytes and Con A-activated spleen cells, with expression in BALB/c greater than that in C57BL/6. GL7 is commonly used as a marker for mouse germinal center B cells. The epitope of GL7 has been identified as a sialic acid glycan moiety called Neu5Ac. This moiety is recognized by CD22.Specifications
| GL7 | |
| 0.5 mg/mL | |
| Monoclonal | |
| Liquid | |
| RUO | |
| PBS with 0.09% sodium azide; pH 7.2 | |
| IgM | |
| Affinity chromatography | |
| Antibody |
| GL-7 (GL7) | |
| Flow Cytometry, Immunohistochemistry (Frozen), Immunoprecipitation | |
| Unconjugated | |
| Rat | |
| Human, Mouse | |
| GL7 antigen; Ly77; Ly-77 | |
| Primary | |
| 4° C |
For Research Use Only.
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