Invitrogen™ Globo H Monoclonal Antibody (VK9), eBioscience™, Invitrogen™

Catalog No.	Quantity
50-112-9168	100 μg

Catalog No. 50-112-9168 Supplier Invitrogen™ Supplier No. 14970082

Description

Mouse Monoclonal Antibody

Description: The VK9 monoclonal antibody recognizes human Globo H, a hexasaccharide formed from the pentasaccharide precursor, SSEA3 (stage-specific embryonic antigen 3). It is a member of a family of antigenic carbohydrates expressed on the surface of cancer cells as glycolipids and possibly glycoproteins. Globo H is overexpressed on many carcinomas, such as breast, colon, ovarian, gastric, pancreatic, lung, and prostate. Expression is observed in more than half of all ductal, lobular, and tubular breast carcinomas, but not in non-epithelial breast tumors. Globo H is also expressed on cancer stem cells and human embryonic stem cells, although expression decreases when cells are differentiated toward both neural progenitor cells and to a lesser extent in endodermal lineage cells. Expression in normal tissues is limited to cells composing secretory borders. Globo H is considered a target for immunotherapy of many epithelial cancers. Applications Reported: This VK9 antibody has been reported for use in flow cytometric analysis, western blotting, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, and microscopy. Applications Tested: This VK9 antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded human tissue using either high or low pH antigen retrieval at less than or equal to 5 μg/mL.

Globo H is a hexasaccharide formed from the pentasaccharide precursor, SSEA3 (stage-specific embryonic antigen 3). It is a member of a family of antigenic carbohydrates expressed on the surface of cancer cells as glycolipids and possibly glycoproteins. Globo H is overexpressed on many carcinomas, such as breast, colon, ovarian, gastric, pancreatic, lung, and prostate. Expression is observed in more than half of all ductal, lobular, and tubular breast carcinomas, but not in non-epithelial breast tumors. Globo H is also expressed on cancer stem cells and human embryonic stem cells, although expression decreases when cells are differentiated toward both neural progenitor cells and to a lesser extent in endodermal lineage cells. Expression in normal tissues is limited to cells composing secretory borders. Globo H is considered a target for immunotherapy of many epithelial cancers.

Specifications

• Antigen: Globo H
• Applications: Flow Cytometry, Immunohistochemistry (Paraffin), Western Blot
• Classification: Monoclonal
• Clone: VK9
• Concentration: 0.5 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with proprietary stabilizer and 0.09% sodium azide; pH 7.2
• Gene Alias: Globo H antigen; globoH; Globohexaosylceramide
• Host Species: Mouse
• Purification Method: Affinity chromatography
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Human
• Content And Storage: 4°C
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG3 κ

For Research Use Only.