Promega GloSensor™ cAMP Assay

Description

Requires

GloSensor cAMP Reagent is required for use with this assay per the GloSensor Limited Use Label License.

The GloSensor cAMP Assay is based on a genetically modified form of firefly luciferase into which a cAMP-binding protein moiety has been inserted. This live-cell assay excels at kinetic and modulation studies of signaling through cAMP.

• Live-Cell Biosensors for cAMP, cGMP or Protease Activity
• Biosensors enable more complete analysis of receptor biology
• Simple, scalable assay protocol
• High Z´ and large signal:background ratio

Specifications

• Product Type: GloSensor cAMP Reagent
• Detection Method: Luminescence
• Content And Storage: <-65°C
• For Use With (Application): Full and partial agonists and antagonists as well as inverse agonists and allosteric modulators
• Quantity: 250 mg
• Form: Fluid

• By use of this product, researcher agrees to be bound by the terms of this limited use statement. If the researcher is not willing to accept the conditions of this limited use statement, and the product is unused, Promega will accept return of the unused product and provide the researcher with a full refund.
• Not for use in live animals—For use in live animals contact Promega regarding the availability of licenses. Researchers may use this product and its derivatives in their own research solely for the luminescent determination of cAMP levels in vitro or within cells (excluding uses in living animals). Researchers shall have no right to modify or otherwise create variations of the nucleotide sequence encoding the GloSensor cAMP Reporter of this product (i.e., the bioluminescent chimeric protein comprising luciferase and cAMP-binding domains). Researchers may transfer derivatives to others provided that recipients agree to be bound by the conditions of this limited use statement prior to such transfer. No other use or transfer of this product or derivatives is authorized without the prior express written consent of Promega. In addition, researchers must either: (1) use the GloSensor cAMP Reagent purchased from Promega for all determinations of luminescence activity of this product and derivatives; or (2) contact Promega to obtain a license for use of the product and derivatives in conjunction with luminescent assay reagents not purchased from Promega. Promega makes no representations or warranties of any kind, either expressed to implied, including for merchantability or fitness for a particular purpose with regards to the product. The terms of this agreement shall be governed under the laws of the State of Wisconsin, USA.
• Mfr. No. E2301, E1171: U.S. Patent 5,670,356.
• Mfr. No. E2301, E1171: Australian Patent 2001 285278 and other patents pending.
• Mfr. No. E1261: Commercial use of this cell line requires a license from AdVec Inc.
• Mfr. No. E1261: HEK293 cells were obtained under license from AdVec Inc.
• Mfr. Nos. E1261, E2301, E1171: The method of recombinant expression of Coleoptera luciferase is covered by U.S. Patents 5,583,024; 5,674,713 and 5,700,673. A license (from Promega for research reagent products and from The Regents of the University of California for all other fields) is needed for any commercial sale of nucleic acid contained within or derived from this product.