MilliporeSigma™ Glucokinase/GCK, Mouse, Unlabeled, Clone: 9G1.2,

Mouse Monoclonal Antibody

Supplier:  MilliporeSigma™ MABS1190

Catalog No. MABS1190MI

Description

Glucokinase (EC 2.7.1.2; UniProt P35557; also known as GK, Hexokinase-4, Hexokinase-D, Hexokinase type IV, HK IV, HK4) is encoded by the GCK (also known as HHF3, MODY2) gene (Gene ID 2645) in human. Glucokinase/GK functions as the body's primary glucose sensor by catalyzing the ATP-dependent phosphorylation of glucose to glucose-6-phosphate, the rate-limiting step of glucose metabolism in human pancreas and liver. GK activity is regulated rapidly in response to the elevated blood glucose after feeding, whereby a change in GK conformation causes a dissociation of the nuclear GK-glucokinase regulatory protein (GKRP) complex, allowing GK translocation to the cytoplasm in an activated state. In mammals, there exist four types of hexokinases, HK1, HK2, and HK3 have high affinity for glucose, while GK (also known as HK4) has low affinity for glucose and its expression is restricted to the pancreas and liver. GCK gene mutations cause Maturity-onset diabetes of the young 2 (MODY2) and Familial hyperinsulinemic hypoglycemia 3 (HHF3).

Specifications

• Antigen: Glucokinase/GCK
• Classification: Monoclonal
• Concentration: Please refer to lot specific datasheet.
• Formulation: Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% Sodium Azide.
• Gene Accession No.: P35557
• Immunogen: GST-tagged recombinant internal fragment from the C-terminal half of human Glucokinase/GCK.
• Quantity: 100 μg
• Research Discipline: Signaling
• Gene ID (Entrez): NP_000153
• Target Species: Human
• Form: Purified

• Applications: Western Blot
• Clone: 9G1.2
• Conjugate: Unconjugated
• Gene: GCK, HHF3, MODY2
• Host Species: Mouse
• Purification Method: Protein G Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: Clone 9G1.2 recognizes an epitope present in all three spliced isoforms of human Glucokinase/GCK reported by UniProt (P35557).
• Content And Storage: Stable for 1 year at 2°-8°C from date of receipt.
• Isotype: IgG1 κ

For Research Use Only. Not for use in diagnostic procedures.