MilliporeSigma™ Glutamine Synthetase, Mouse, Unlabeled, Clone: 11A3.1,

Catalog No.	Quantity	Conjugate
MABN1182MI	100 μL	Unconjugated

Catalog No. MABN1182MI Supplier MilliporeSigma™ Supplier No. MABN1182

Description

Mouse Monoclonal Antibody

Glutamine synthetase (EC 4.1.1.15, EC 6.3.1.2; UniProt P09606; also known as Glutamate-ammonia ligase, Glutamate decarboxylase, GS) is encoded by the Glul (also known as Glns) gene (Gene ID 24957) in rat species. Glutamine synthetase (GS) plays an essential role in nitrogen metabolism by catalyzing ATP-dependent condensation of glutamate and ammonia to form glutamine. Three classes of GS exist, class I enzymes (GSI) are specific to prokaryotes and exist as oligomers of 12 identical subunits, class II enzymes (GSII) exist as decamer of identical subunits in eukaryotes and in some bacteria, while class III enzymes (GSIII) function as a double-ringed dodecamer of identical chains found in bacteroides fragilis and in butyrivibrio fibrisolvens. GS is present predominantly in the brain, kidneys, and liver in mammals. In the brain, GS is found primarily in astrocytes, where it participates in the the detoxification of ammonia via assimilation and termination of neurotransmitter signals via recyclization. c-Myc promotes glutamine usage by upregulating glutaminase (GLS), which converts glutamine to glutamate in the TCA cycle, while in some cancer cells Myc-mediated thymine DNA glycosylase (TDG) transcription leads to GS upregulation due to TDG-dependent demethylation of the GS promoter. GS upregulation promotes cancer cell survival under glutamine limitation, while GS silencing decreases cancer cell proliferation and xenograft tumor growth.

Specifications

• Antigen: Glutamine Synthetase
• Applications: Western Blot
• Classification: Monoclonal
• Clone: 11A3.1
• Concentration: Please refer to lot specific datasheet.
• Conjugate: Unconjugated
• Formulation: Purified mouse monoclonal IgG2aκ in buffer containing 0.1M Tris-Glycine (pH 7.4) 150mM NaCl with 0.05% Sodium Azide.
• Gene: Glul, Glns
• Gene Accession No.: P09606
• Host Species: Mouse
• Immunogen: GST-tagged recombinant rat glutamine synthetase internal fragment.
• Purification Method: Protein G Purified
• Quantity: 100 μL
• Regulatory Status: RUO
• Research Discipline: Neuroscience
• Primary or Secondary: Primary
• Gene ID (Entrez): NP_058769
• Test Specificity: Clone 11A3.1 targets an internal epitope within the C-terminal half of human and rat glutamine synthetase.
• Target Species: Human
• Content And Storage: Stable for 1 year at 2°-8°C from date of receipt.
• Form: Purified
• Isotype: IgG2a κ

For Research Use Only. Not for use in diagnostic procedures.