MilliporeSigma™ Glycoprotein 78, Rat, Unlabeled, Clone: 3F3A,

Catalog No.	Quantity	Conjugate
MABC949	200 μL	Unconjugated

Catalog No. MABC949 Supplier MilliporeSigma™ Supplier No. MABC949

Description

Rat Monoclonal Antibody

E3 ubiquitin-protein ligase AMFR (UniProt Q9UKV5; also known as AMF receptor, Autocrine motility factor receptor, gp78, RING finger protein 45) is encoded by the RNF45 (also known as AMFR) gene (Gene ID 267) in human. Glycoprotein 78 (gp78) is an endoplasmic reticulum (ER) membrane-anchored E3 ubiquitin ligase that plays a key role in ER-associated degradation (ERAD). Gp78 is localized to a mitochondria-associated ER domain, where it induces mitochondrial fragmentation by targeting the mitochondrial fusion proteins mitofusin 1 and 2 (Mfn1 and Mfn2, respectively) for degradation upon mitochondrial depolarization. Other known ERAD substrates processed by gp78 include ApoB lipoprotein, HMG CoA reductase, CD3-delta, cystic fibrosis transmembrane conductance regulator, the metastasis suppressor KAI1, unglycosylated prion protein (PrP), and the T-cell receptor. Gp78 is a 7-transmembrane protein (a.a. 82-102, 122-142, 145-165, 186-206, 215-235, 276-296, 429-449) with a RING-type Zinc finger (a.a. 341-379), a CUE domain (a.a. 456-498), and a VCP/p97-interacting motif (VIM; a.a. 622-640).

Specifications

• Antigen: Glycoprotein 78
• Applications: Immunocytochemistry, Immunohistochemistry, Western Blot
• Classification: Monoclonal
• Clone: 3F3A
• Concentration: Please refer to lot specific datasheet.
• Conjugate: Unconjugated
• Formulation: Purified rat IgM in buffer containing PBS without azide.
• Gene: RNF45, AMFR
• Gene Accession No.: Q9UKV5
• Host Species: Rat
• Immunogen: Peanut lectin (PNA) resin-purified membrane glycoprotein 78 from B16-F1 cells (Nabi, I.R., and Raz, A. (1987). Int. J. Cancer. 40(3):396-402).
• Purification Method: Purified by Ion-Exchange Chromatography
• Quantity: 200 μL
• Regulatory Status: RUO
• Research Discipline: Apoptosis & Cancer
• Primary or Secondary: Primary
• Gene ID (Entrez): NP_001135
• Test Specificity: Clone 3F3A detected glycoprotein 78 (gp78) and a ∽150 kDa glycoprotein in B16-F1 mouse melanoma cell extract. Cell extract sialidase treatment decreased the antibody's immunoreactivity toward gp78 and abolished ∽150 kDa band detection (Nabi, I.R., et al. (1990). Cancer Res. 50(2):409-414).
• Target Species: Human
• Content And Storage: Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
• Form: Purified
• Isotype: IgM κ

For Research Use Only. Not for use in diagnostic procedures.