Novus Biologicals™ GM-CSF Antibody Pair [HRP]

Polyclonal/Monoclonal Antibody Pair

Supplier:  Novus Biologicals™ NBP27960915PLATES

Catalog No. NB010257

Description

This solid phase sandwich ELISA utilizes an antibody specific for Mouse GM-CSF.

Specifications

• Assay Range: 1.88 pg/mL
• Assay: Sandwich ELISA
• Immunoassay Kit Format: Sandwich ELISA Kit
• Specificity: This solid phase sandwich ELISA utilizes an antibody specific for Mouse GM-CSF.
• For Use With (Application): Sandwich ELISA
• Gene ID (Entrez): 1437
• Host Species: Rabbit
• Quantity: 15 Plates
• Research Discipline: Biologically Active Proteins, Cytokine Research, Immunology
• Target: GM-CSF

• Assay Sensitivity: 1.88-120 pg/mL
• Conjugate: HRP
• Product Type: Antibody Pairs
• Target Species: Mouse
• Gene Alias: colony stimulating factor 2 (granulocyte-macrophage), Colony-stimulating factor, CSF, GM-CSF, GMCSFgranulocyte-macrophage colony-stimulating factor, granulocyte-macrophage colony stimulating factor, MGC131935, MGC138897, molgramostin, sargramostim
• Gene Symbol: CSF2
• Kit Contents:
• Detection Antibody: 0.2 mg/mL of rabbit anti-GM-CSF polyclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4C).
• Capture Antibody: 1 mg/mL of rabbit anti-Mouse GM-CSF monoclonal antibody (in PBS, pH 7.4).
• Standard: Each vial contains 11 ng of recombinant Mouse GM-CSF.
• Regulatory Status: RUO
• Storage Requirements: Storage is content dependent.
• Technique: 1. Capture Antibody: Dilute to a working concentration of 2 μg/mL in PBS before coating. 2. Detection Antibody: Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use. 3. Standard: Reconstitute with 1 ml detection antibody dilution buffer. After reconstitution, store at -20 to -80°C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 120 pg/mL is recommended.