Invitrogen™ GM-CSF Monoclonal Antibody (31G6), Biotin

Rat Monoclonal Antibody

Supplier:  Invitrogen™ MM500DB

Catalog No. ENMM500DB

Description

MM500DB targets Mouse GM-CSF in WB applications and shows reactivity with mouse samples. The MM500DB immunogen is recombinant mouse GM-CSF. MM500DB detects Mouse GM-CSF which has a predicted molecular weight of approximately 14 kDa. The MM500DB mouse GMCSF antibody (biotinylated conjugate of clone 31G6) has successfully been paired as the detection antibody in a sandwich ELISA with capture antibody MM500C (clone 22E9) Typical dilutions for sandwich ELISA include 1 μg/mL for coating and 0.125 - 0.25 μg/mL for detection. Biotinylated antibody MM500DB (clone 31G6) and antibody MM500C (clone 22E9) have successfully been used in combination with recombinant mouse GMCSF protein SMGMCSF in ELISA applications. This antibody is produced by injecting Rat IgG secreting hybridoma cells into the peritoneum of mice. The resulting ascites is collected from the mice and the antibody is purified.

The GM-CSF gene encodes for a cytokine that regulates the production, differentiation, and function of macrophages and granulocytes. The active form of the protein exists as a homodimer in the extracellular space. This gene is located in a cluster of related genes at the chromosome region 5q31, which has been associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include interleukins 4, 5, and 13. The gene is involved in promoting tissue inflammation. Elevated levels of cytokines, including the one produced by this gene, have been observed in SARS-CoV-2 infected patients with acute respiratory distress syndrome. Mice that lack this gene or its receptor have been shown to develop pulmonary alveolar proteinosis.

Specifications

• Antigen: GM-CSF
• Classification: Monoclonal
• Concentration: 0.5 mg/mL
• Formulation: PBS with 4% BSA and proprietary Preservative
• Gene Accession No.: P01587
• Gene Symbols: CSF2
• Immunogen: Recombinant mouse GM-CSF.
• Quantity: 250 μg
• Primary or Secondary: Primary
• Target Species: Mouse
• Product Type: Antibody
• Isotype: IgG1

• Applications: Western Blot
• Clone: 31G6
• Conjugate: Biotin
• Gene: CSF2
• Gene Alias: colony stimulating factor 2; colony stimulating factor 2 (granulocyte-macrophage); colony-stimulating factor; CSF; CSF2; Csfgm; cytokine; GMCSF; Gm-CSf; granulocyte macrophage colony stimulating factor; granulocyte macrophage-colony stimulating factor; granulocyte-macrophage colony stimulating factor 2; granulocyte-macrophage colony-stimulating factor; MGC131935; MGC138897; MGC151255; MGC151257; MGI-IGM; M-GM-CSF; molgramostin; put. GM-CSF; sargramostim
• Host Species: Rat
• Purification Method: Protein G
• Regulatory Status: RUO
• Gene ID (Entrez): 12981
• Content And Storage: -20°C
• Form: Liquid