GM-CSF Rat anti-Mouse, Functional Grade, Clone: MP1-22E9, eBioscience™

Rat Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
50-147-56	Invitrogen 16733185	500 μg
16-733-181	Invitrogen 16733181	50 μg

Description

Description: The MP1-22E9 antibody reacts with mouse granulocyte/macrophage - colony stimulating factor (GM-CSF). The MP1-22E9 antibody is a neutralizing antibody. Mouse GM-CSF is a 14 kDa factor produced mainly by activated T cells and macrophages. Other cell types, such as endothelium and fibroblasts, also secrete GM-CSF in response to TNF-alpha, IL-2, IL-1, and IFN-gamma. GM-CSF stimulates growth of macrophages, granulocytes and dendritic cells. GM-CSF is found as a membrane-bound form and also as a complex associated with the extracellular matrix. Non-glycosylated GM-CSF is biologically active. Applications Reported: The MP1-22E9 antibody has been reported for use in ELISA, ELISPOT, neutralization, and intracellular staining for flow cytometric analysis. Applications Tested: The Functional Grade Purified MP1-22E9 antibody has been tested by LAL assay to verify low endotoxin levels, by ELISA, and in bioassay for neutralization of GM-CSF bioactivity. The MP1-22E9 antibody at 1.25 µg/mL has been found to inhibit by 50% the biological effects of 1 ng/mL mouse GM-CSF in an MC/9 cell proliferation assay. Detailed information and protocols about cytokine bioassays and in vitro cytokine neutralization using antibodies can be found in the BestProtocols™ section.

The MP1-22E9 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of mouse GM-CSF (Granulocyte/Macrophage-Colony Stimulating Factor, GMCSF) in combination with the biotin MP1-31G6 (13-7332) antibody for detection and recombit mouse GM-CSF (14-8331) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 1-4 µg/mL. A standard curve consisting of doubling dilutions of the recombit standard over the range of 1000 pg/mL - 8 pg/mL should be included in each ELISA plate. Endotoxin: Less than 0.001 ng/ug antibody as determined by the LAL assay. Storage and handling: Use in a sterile environment. Filtration: 0.2 μm post-manufacturing filtered. Purity: Greater than 90%, as determined by SDS-PAGE. Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay. Aggregation: Less than 10%, as determined by HPLC. GM-CSF (Granulocyte-Macrophage colony-stimulating factor) is a 14.6kDa hematopoietic growth factor that exists in glycosylated and non-glycosylated biologically active forms, and stimulates the development of granulocytes, macrophages, early megakaryocytes and eosinophil progenitor cells. The active form of the GM-CSF protein is found extracellularly as a homodimer and the GM-CSF gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q-syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13. The ability of recombit GM-CSF to increase hematopoietic cell recovery has become a focus area in the therapeutic treatment of patients following bone marrow transplantation. Recent studies have investigated GM-CSF in inflammatory and autoimmune diseases such as arthritis, arthritis related interstitial lung disease, nephritis, and psoriasis. In the CNS, GM-CSF depletion or neutralization has been studied in experimental autoimmune encephalomyelitis (EAE) and multiple sclerosis (MS).

Specifications

• Antigen: GM-CSF
• Target Species: Mouse
• Isotype: IgG2a κ
• Primary or Secondary: Primary
• Classification: Monoclonal
• Formulation: PBS with no preservative; pH 7.2
• Regulatory Status: RUO

• Clone: MP1-22E9
• Conjugate: Functional Grade
• Applications: ELISA, Functional Assay, Neutralization
• Form: Liquid
• Product Type: Antibody
• Content And Storage: 4° C

For Research Use Only.