GNAI3 Polyclonal Antibody, Invitrogen™

Rabbit Polyclonal Antibody

Supplier:  Thermo Scientific PA527940

Catalog No. PIPA527940

Description

Recommended positive controls: 293T, A431, H1299, HeLaS3, HepG2, Molt-4, Raji, mouse brain. Predicted reactivity: Mouse (97%), Rat (97%), Zebrafish (88%), Japanese Medaka (91%), Xenopus laevis (93%), Pig (99%), Chicken (97%), Rhesus Monkey (100%), Bovine (98%), Guinea pig (97%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Heterotrimeric G proteins function to relay information from cell surface receptors to intracellular effectors. Each of a very broad range of receptors specifically detects an extracellular stimulus (a photon, pheromone, odorant, hormone or neurotransmitter) while the effectors (i. e. adenyl cyclase), which act to generate one or more intracellular messengers, are less numerous. In mammals, G protein alpha, beta and gamma polypeptides are encoded by at least 16, 4 and 7 genes, respectively. Most interest in G proteins has been focused on their alpha subunits, since these proteins bind and hydrolyze GTP and most obviously regulate the activity of the best studied effectors. Four distinct classes of Galpha subunits have been identified; these include Gs, Gi, Gq and Galpha 12/13. The Gi class comprises all the known alpha subunits that are susceptible to pertussis toxin modifications, including Galpha i-1, Galpha i-2, Galpha i-3, Galpha o, Galpha t1, Galpha t2, Galpha z and Galpha gust. Of these, the three Galpha i subtypes function to open atrial potassium channels.

Specifications

• Antigen: GNAI3
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: GNAI3
• Gene Alias: 87U6; AI158965; ARCND1; AW537698; G protein subunit alpha i3; g(i) alpha-3; Galphai3; GIP3A; GNAI3; Gnai-3; guanine nucleotide binding protein (G protein), alpha inhibiting 3; guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 3; guanine nucleotide binding protein, alpha inhibiting 3; Guanine nucleotide binding, protein, alpha inhibiting polypeptide 3; Guanine nucleotide-binding protein G(i) subunit alpha; guanine nucleotide-binding protein G(k) subunit alpha
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 14679, 2773
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot
• Concentration: 0.94 mg/mL
• Formulation: 0.1M tris glycine with 10% glycerol and 0.01% thimerosal; pH 7
• Gene Accession No.: P08754, Q9DC51
• Gene Symbols: GNAI3
• Immunogen: Recombinant fragment corresponding to a region within amino acids 68 and 354 of Human G protein alpha Inhibitor 3
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgG