MilliporeSigma™ Goα, Mouse, Unlabeled, Clone: 3D9.1,

Catalog No.	Quantity	Conjugate
MABS1691	100 μL	Unconjugated

Catalog No. MABS1691 Supplier MilliporeSigma™ Supplier No. MABS1691

Description

Mouse Monoclonal Antibody

Guanine nucleotide-binding protein G(o) subunit alpha (UniProt P29777; also known as G(o)alpha) is encoded by the GNAO1 (also known as EIEE17) gene (Gene ID 2775) in human. Heterotrimeric G proteins are activated by G protein-coupled receptors (GPCRs) and are composed of alpha, beta, and gamma subunits. There exists also monomeric Ras superfamily of small GTPases that are commonly referred to as small G-proteins. G-proteins mediate signal transduction initiated by GPCRs. Ligand binding activates GPCRs by inducing a conformational change. The activated GPCRs catalyzes the GDP-to-GTP exchange of the G subunit, causing a dissociation of the G dimer from the now GTP-bound G subunit. The G subunit gradually hydrolyzes the bound GTP to GDP and eventually re-associates with G and re-starts the cycle. There exist at least 20 different G subunits, which are separated into four main groups, Gi, Gs, Gq, and G12/13. Five types of Gi alpha subunits exist, Gi (GNAI1, GNAI2, and GNAI3), Go (GNAO1), Gt (GNAT1 and GNAT2), Ggust (GNAT3), and Gz (GNAZ). Gi/o family members inhibit adenylyl cyclase and can reduce neuronal excitability through regulation of ion channels. G(o)alpha plays a key role in presynaptic inhibition of neurotransmitter release through a G-mediated suppression of voltage-gated Ca2+ channels. G(o)alpha is the most abundant G o protein in the mammalian brain, defective G(o)alpha function can result in neurological impairments.

Specifications

• Antigen: Goα
• Applications: Western Blot
• Classification: Monoclonal
• Clone: 3D9.1
• Concentration: Please refer to lot specific datasheet.
• Conjugate: Unconjugated
• Formulation: Mouse monoclonal hybridoma culture supernatant without preservatives.
• Gene: GNAO1, EIEE17
• Gene Accession No.: P29777
• Host Species: Mouse
• Immunogen: KLH-conjugated linear peptide corresponding to a sequence from the C-terminal region of human Go (G(o)alpha).
• Purification Method: Unpurified
• Quantity: 100 μL
• Regulatory Status: RUO
• Research Discipline: Signaling
• Primary or Secondary: Primary
• Gene ID (Entrez): NP_066268
• Test Specificity: Clone 3D9.1 was raised against a C-terminal region sequence derived from human Go (G(o)alpha) spliced isoform Alpha-1. Cross-reactivity toward human/mouse/rat spliced isoform Alpha-2 (∽69% amino acid identity with spliced isoform Alpha-1) has not been confirmed.
• Target Species: Mouse
• Content And Storage: Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
• Form: Culture Supernatant
• Isotype: IgG1 κ

For Research Use Only. Not for use in diagnostic procedures.