NKX2-5, Goat, Polyclonal Antibody, Abnova™

Catalog No.	Quantity
89-117-036	100 μg

Catalog No. 89-117-036 Supplier Abnova Corporation Supplier No. PAB14394

Description

Goat polyclonal antibody raised against synthetic peptide of NKX2-5.

Homeobox-containing genes play critical roles in regulating tissue-specific gene expression essential for tissue differentiation, as well as determining the temporal and spatial patterns of development (Shiojima et al., 1995 [PubMed 7665173]). It has been demonstrated that a Drosophila homeobox-containing gene called 'tinman' is expressed in the developing dorsal vessel and in the equivalent of the vertebrate heart. Mutations in tinman result in loss of heart formation in the embryo, suggesting that tinman is essential for Drosophila heart formation. Furthermore, abundant expression of Csx, the presumptive mouse homolog of tinman, is observed only in the heart from the time of cardiac differentiation. CSX, the human homolog of murine Csx, has a homeodomain sequence identical to that of Csx and is expressed only in the heart, again suggesting that CSX plays an important role in human heart formation.[supplied by OMIM

Sequence: C-PRAYSDPDPAKDPR

Specifications

• Antigen: NKX2-5
• Applications: ELISA, Western Blot
• Classification: Polyclonal
• Concentration: 0.5 mg/mL
• Conjugate: Unconjugated
• Description: Goat polyclonal antibody raised against synthetic peptide of NKX2-5.
• Dilution: ELISA (1:32000) Western Blot (0.3-1 ug/mL) The optimal working dilution should be determined by the end user.
• Formulation: In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
• Gene: NKX2-5
• Gene Alias: CHNG5/CSX/CSX1/NKX2.5/NKX2E/NKX4-1
• Gene Symbols: NKX2-5
• Host Species: Goat
• Immunogen: A synthetic peptide corresponding to human NKX2-5.
• Purification Method: Antigen affinity purification
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 1482
• Test Specificity: Approx 45 KDa band observed in human heart lysates (calculated MW of 34.9 KDa according to NP_004378.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Shiojima et al, Circ Res. 1996 Nov;79(5):920-9.; PMID: 8888684).
• Target Species: Human, Mouse, Rat
• Content And Storage: Store at -20°C.
  Aliquot to avoid repeated freezing and thawing.
• Form: Liquid

For Research Use Only