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Gold nanoparticles, 20nm, supplied in 0.1 mg/ml sodium citrate with stabilizer, OD1, 524nm absorption
Description
Colloidal gold is an electron-dense, non-fading marker useful as a probe in electron microscopy, light microscopy and immunoblotting. It requires no additional processing for detection, but in some applications the signal can be enhanced by reaction with silver. It can be complexed with biomolecules by strong, non-covalent interactions. Particles less than 15 nm are most useful in transmission electron microscopy and for applications where access to the probe may be hindered by larger particles.
This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.
Colloidal gold is an electron-dense, non-fading marker useful as a probe in electron microscopy, light microscopy and immunoblotting. It requires no additional processing for detection, but in some applications the signal can be enhanced by reaction with silver. It can be complexed with biomolecules by strong, non-covalent interactions. Particles less than 15 nm are most useful in transmission electron microscopy and for applications where access to the probe may be hindered by larger particles.
Solubility
Fully miscible in water. It is soluble in aqua regia, nitric acid and hydrochloric acid.
Notes
Particles per ml: 6.54E+11
Specifications
Specifications
| Color | Pink to Red |
| Quantity | 25 mL |
| Particle Size | 20nm |
| Sensitivity | Light sensitive |
| Form | Liquid |
| Solubility Information | Fully miscible in water. It is soluble in aqua regia,nitric acid and hydrochloric acid. |
| Chemical Name or Material | Gold nanoparticles, supplied in 0.1mg/mL sodium citrate with stabilizer, OD: 1, 524nm absorption |
Frequently Asked Questions (FAQs)
Irreversible aggregation is characterized by a clear to bluish solution upon the addition of salt. Since non-functionalized gold nanoparticles are sensitive to salt-containing buffers, re-suspension should always be performed in ultra-pure water to prevent irreversible aggregation.
Although it is not generally necessary to wash the gold nanoparticles prior to use, some applications may require additional washing procedures. The easiest way to remove possible contaminants in the nanoparticles solution is by centrifugation. Centrifugation force is dependent on size of the gold nanoparticles. We recommend the following to wash gold nanoparticles:
1. Place 1 mL aliquot of colloidal gold in a 1.5 mL microcentrifuge tube.
2. Centrifuge the gold nanoparticles for 30 minutes using the recommended G force based on particle size (see below).
3. Remove the supernatant and re-suspend in an appropriate volume of ultra-pure water.
4. Vortex to re-disperse the particles.
Centrifugation speed guide for nanoparticle size:
size - G force
5 nm - 100,000 g
10 nm - 17,000 g
15 nm - 17,000 g
20 nm - 6,500 g
30 nm - 4,500 g
40 nm - 2,500 g
50 nm - 2,000 g
60 nm - 1,125 g
80 nm - 600 g
100 nm - 400 g
Although it is not generally necessary to wash the gold nanoparticles prior to use, some applications may require additional washing procedures. The easiest way to remove possible contaminants in the nanoparticles solution is by centrifugation. Centrifugation force is dependent on size of the gold nanoparticles. We recommend the following to wash gold nanoparticles:
1. Place 1 mL aliquot of colloidal gold in a 1.5 mL microcentrifuge tube.
2. Centrifuge the gold nanoparticles for 30 minutes using the recommended G force based on particle size (see below).
3. Remove the supernatant and re-suspend in an appropriate volume of ultra-pure water.
4. Vortex to re-disperse the particles.
Centrifugation speed guide for nanoparticle size:
size - G force
5 nm - 100,000 g
10 nm - 17,000 g
15 nm - 17,000 g
20 nm - 6,500 g
30 nm - 4,500 g
40 nm - 2,500 g
50 nm - 2,000 g
60 nm - 1,125 g
80 nm - 600 g
100 nm - 400 g
We recommend 4°C storage for our Gold nanoparticles that are supplied in 0.1 mg/ml sodium citrate with stabilizer. Do not freeze the particles because this causes irreversible aggregation. When stored for a long period of time, gold nanoparticles may sediment at the bottom of the flask, which is especially true for larger particle sizes. Prior to use, re-suspend the sedimented particles by swirling until a homogenous solution is obtained.
Safety and Handling
| TSCA | Yes |
| storageNote1 | 2° to 8°C |
| Recommended Storage | Keep cold |
RUO – Research Use Only
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