Golgi Glycoprotein 1/GLG1 Antibody (GLG1/970), Alexa Fluor™ 750, Novus Biologicals™

Mouse Monoclonal Antibody

Supplier:  Novus Biologicals NBP247794AF750

Catalog No. NB007384

Description

Golgi Glycoprotein 1/GLG1 Monoclonal specifically detects Golgi Glycoprotein 1/GLG1 in Human, Mouse (Negative), Rat (Negative) samples. It is validated for Immunohistochemistry, Immunohistochemistry-Paraffin.

Specifications

• Antigen: Golgi Glycoprotein 1/GLG1
• Classification: Monoclonal
• Conjugate: Alexa Fluor 750
• Formulation: 50mM Sodium Borate with 0.05% Sodium Azide
• Gene Symbols: GLG1
• Immunogen: Golgi fraction from human liver cells
• Quantity: 0.1 mL
• Primary or Secondary: Primary
• Target Species: Human, Mouse (Negative), Rat (Negative)
• Isotype: IgG1 κ

• Applications: Western Blot, Flow Cytometry, Immunohistochemistry, Immunohistochemistry (Paraffin), Immunofluorescence
• Clone: GLG1/970
• Dilution: Western Blot, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunofluorescence, Immunocytochemistry
• Gene Alias: CFR1, CFR-1, cysteine-rich fibroblast growth factor receptor, DKFZp686L08213, E-selectin ligand 1, ESL1, ESL-1, FLJ23319, FLJ23967, FLJ32797, Golgi apparatus protein 1, golgi glycoprotein 1, golgi sialoglycoprotein MG-160, MG160, MG-160
• Host Species: Mouse
• Purification Method: Protein A or G purified
• Regulatory Status: RUO
• Test Specificity: This monoclonal antibody recognizes a protein of 134kDa, which binds fibroblast growth factor and E-selectin (cell-adhesion lectin on endothelial cells mediating the binding of neutrophils). Fucosylation is essential for binding to E-selectin. It contains sialic acid residues and 16 Cys-rich GLG1 repeats. This monoclonal antibody can be used to stain the Golgi complex in cell or tissue preparations and can be used as a Golgi marker in subcellular fractions. It produces a diffuse staining pattern of the Golgi zone in normal and malignant cells. This monoclonal antibody is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells. The Golgi apparatus is an organelle present in all eukaryotic cells that forms a part of the endomembrane system. The primary function of the Golgi apparatus is to process and package macromolecules synthesized by the cell for exocytosis or use within the cell. The Golgi is made up of a stack of flattened, membrane-bound sacs known as cisternae, with three functional regions: the cis face, medial region and trans face. Each region consists of various enzymes that selectively modify the macromolecules passing though them, depending on where they are destined to reside. Several spherical vesicles that have budded off of the Golgi are present surrounding the main cisternae. The Golgi tends to be more pronounced and numerous in cells that make and secrete many substances such as plasma B cells.
• Content And Storage: Store at 4°C in the dark.