Invitrogen™ GOLPH3 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA532047

Catalog No. PIPA532047

Description

Recommended positive controls: Mouse lung. Predicted reactivity: Mouse (99%), Rat (99%), Zebrafish (90%), Xenopus laevis (89%), Bovine (97%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

GOLPH3 was initially identified as a peripheral membrane protein localized to the trans-Golgi network, but others reported it to be primarily a mitochondrial protein that regulated the mitochondrial mass through the regulation of the mitochondria-specific phospholipid cardiolipin. GOLPH3 has since been implicated in the target of rapamycin (TOR) signaling pathway. Its overexpression in transfected cells led to and increase in anchorage-independent growth and cell proliferation in vitro. Furthermore, GOLPH3-transfected cells enhanced S6 Kinase activity in response to growth factor stimulation by EGF. Simultaneously, AKT phosphorylation increased in these cells, while these events were abrogated in GOLPH3 siRNA treated cells compared to control cells, indicating the GOLPH3 can enhance signaling through TOR-associated complexes. These results suggest that GOLPH3 is a bona fide oncogene and may be a useful target for therapeutic strategies.

Specifications

• Antigen: GOLPH3
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: Golph3
• Gene Alias: 4733401N08Rik; 5730410D03Rik; AW413496; coat protein GPP34; coat-protein; Gmx33; golgi peripheral membrane protein 1, 34 kDa; Golgi phosphoprotein 3; golgi phosphoprotein 3 (coat-protein); golgi protein; golgi-associated protein; GOLPH3; GOPP1; GPP34; MIDAS; Mitochondrial DNA absence factor; trans-Golgi protein GMx33; Vps74
• Host Species: Rabbit
• Purification Method: Antigen Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 64083, 66629
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Western Blot
• Concentration: 1 mg/mL
• Formulation: PBS with 1% BSA, 20% glycerol and 0.01% thimerosal; pH 7
• Gene Accession No.: Q9CRA5, Q9H4A6
• Gene Symbols: Golph3
• Immunogen: Recombinant fragment corresponding to a region within amino acids 36 and 298 of Human GOLPH3.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgG