Invitrogen™ GOLPH3 Polyclonal Antibody

Catalog No.	Quantity
PIPA565982	100 μL

Catalog No. PIPA565982 Supplier Invitrogen™ Supplier No. PA565982

Description

Rabbit Polyclonal Antibody

Immunogen sequence: GSSEDDAQSRR DEQDDDDKGD SKETRLTLME E Highest antigen sequence identity to the following orthologs - mouse 94%, rat 94%.

GOLPH3 was initially identified as a peripheral membrane protein localized to the trans-Golgi network, but others reported it to be primarily a mitochondrial protein that regulated the mitochondrial mass through the regulation of the mitochondria-specific phospholipid cardiolipin. GOLPH3 has since been implicated in the target of rapamycin (TOR) signaling pathway. Its overexpression in transfected cells led to and increase in anchorage-independent growth and cell proliferation in vitro. Furthermore, GOLPH3-transfected cells enhanced S6 Kinase activity in response to growth factor stimulation by EGF. Simultaneously, AKT phosphorylation increased in these cells, while these events were abrogated in GOLPH3 siRNA treated cells compared to control cells, indicating the GOLPH3 can enhance signaling through TOR-associated complexes. These results suggest that GOLPH3 is a bona fide oncogene and may be a useful target for therapeutic strategies.

Specifications

• Antigen: GOLPH3
• Applications: Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Classification: Polyclonal
• Concentration: 0.2 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 40% glycerol and 0.02% sodium azide; pH 7.2
• Gene: Golph3
• Gene Accession No.: Q9H4A6
• Gene Alias: 4733401N08Rik; 5730410D03Rik; AW413496; coat protein GPP34; coat-protein; Gmx33; golgi peripheral membrane protein 1, 34 kDa; Golgi phosphoprotein 3; golgi phosphoprotein 3 (coat-protein); golgi protein; golgi-associated protein; GOLPH3; GOPP1; GPP34; MIDAS; Mitochondrial DNA absence factor; trans-Golgi protein GMx33; Vps74
• Gene Symbols: Golph3
• Host Species: Rabbit
• Immunogen: Recombinant Human GOLPH3. Recombinant protein control fragment (Product #RP-105028).
• Purification Method: Antigen affinity chromatography
• Quantity: 100 μL
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 64083
• Target Species: Human
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG