Novus Biologicals™ gp130/CD130 Antibody Pair [HRP]

Description

Rabbit Monoclonal Antibody Pair

This solid phase sandwich ELISA utilizes a monoclonal antibody specific for Mouse gp130/CD130.

Specifications

• Assay Range: 11.72 pg/mL
• Assay Sensitivity: 11.72-750 pg/mL
• Assay: Sandwich ELISA
• Conjugate: HRP
• Product Type: Antibody Pairs
• Specificity: This solid phase sandwich ELISA utilizes a monoclonal antibody specific for Mouse gp130/CD130.
• Target Species: Mouse
• For Use With (Application): Sandwich ELISA
• Gene Alias: CD130, CD130 antigen, CDw130, DKFZp564F053, gp130, gp130 of the rheumatoid arthritis antigenic peptide-bearing soluble form, IL-6 receptor subunit beta, IL-6R subunit beta, IL-6RB, IL-6R-beta, interleukin 6 signal transducer (gp130, oncostatin M receptor), interleukin receptor beta chain, interleukin-6 receptor subunit beta, Interleukin-6 signal transducer, Membrane glycoprotein 130, membrane glycoprotein gp130, Oncostatin-M receptor subunit alpha
• Gene ID (Entrez): 3572
• Gene Symbol: IL6ST
• Host Species: Rabbit
• Kit Contents:
  Detection Antibody: 0.2 mg/mL rabbit anti-gp130/CD130 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4C).
  Capture Antibody: 1.0 mg/mL of rabbit anti-mouse gp130/CD130 monoclonal antibody (in PBS, pH 7.4).
  Standard: Each vial contains 60 ng of recombinant mouse gp130/CD130.
• Quantity: 15 Plates
• Regulatory Status: RUO
• Research Discipline: Cancer, Immunology
• Storage Requirements: Storage is content dependent.
• Target: gp130/CD130
• Technique: 1. Capture Antibody: Dilute to a working concentration of 2 μg/mL in PBS before coating. 2. Detection Antibody: Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use.. 3. Standard: Reconstitute with 1 ml detection antibody dilution buffer. After reconstitution, store at -20 to -80°C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 750 pg/mL is recommended.