gp96/HSP90B1/GRP94 Antibody (SPM249), Novus Biologicals™

Rat Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
NBP24469000	Novus Biologicals NBP2446900.02MG	0.02 mg
NBP24469001	Novus Biologicals NBP2446900.1MG	0.1 mg
NBP24469002	Novus Biologicals NBP2446900.2MG	0.2 mg

Description

Ensure accurate, reproducible results in Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence

gp96/HSP90B1/GRP94 Monoclonal specifically detects gp96/HSP90B1/GRP94 in Human, Mouse, Rat, Porcine, Bovine, Canine, Chicken, Equine, Guinea Pig, Hamster, Monkey, Rabbit, Sheep, Xenopus samples. It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin.

Specifications

• Antigen: gp96/HSP90B1/GRP94
• Concentration: 0.2mg/mL
• Applications: Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence
• Conjugate: Unconjugated
• Host Species: Rat
• Research Discipline: Cancer, Cellular Markers, ER Markers, Golgi Apparatus Markers, Hypoxia, Membrane Trafficking and Chaperones
• Formulation: 10mM PBS and 0.05% BSA with 0.05% Sodium Azide
• Gene ID (Entrez): 7184
• Immunogen: Purified glucose regulated protein 94 (grp94) from chicken oviducts
• Primary or Secondary: Primary
• Content And Storage: Store at 4C.
• Molecular Weight of Antigen: 94 kDa

• Clone: SPM249
• Dilution: Western Blot 0.5 - 1.0 ug/ml, Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml, Immunofluorescence 0.5 - 1.0 ug/ml
• Classification: Monoclonal
• Form: Purified
• Regulatory Status: RUO
• Target Species: Human, Mouse, Rat, Pig, Canine, Chicken, Equine, Guinea Pig, Hamster, Monkey, Primate, Rabbit, Sheep, Xenopus species
• Gene Alias: 94 kDa glucose-regulated protein, endothelial cell (HBMEC) glycoprotein, glucose regulated protein, 94 kDa, GP96ECGP, GRP-94, GRP94gp96 homolog, Heat shock protein 90 kDa beta member 1, heat shock protein 90kDa beta (Grp94), member 1, TRA1endoplasmin, tumor rejection antigen (gp96) 1, Tumor rejection antigen 1, Tumor rejection antigen-1 (gp96)
• Gene Symbols: HSP90B1
• Isotype: IgG2a κ
• Purification Method: Protein A or G purified
• Test Specificity: Recognizes a protein of 94kDa, which is identified as the glucose-regulated protein 94 (grp94) and also tumor rejection antigen (gp96). Grp94 shows a high degree of sequence homology with the heat shock protein 90 (hsp90). This MAb is highly specific to grp94 and shows minimal cross-reaction with other members of the HSP90 family. Grp s are a class of proteins unresponsive to heat shock and are induced by glucose deprivation. Grp94 has been briefly studied as a prognostic factor in breast cancer.

For Research Use Only