GPR109 Monoclonal Antibody (4NZBRGO), PE, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Supplier:  Invitrogen 12477942

Catalog No. 12477942

Description

This 4NZBRGO antibody has not been tested for reactivity with human GPR109B. Applications Reported: This 4NZBRGO antibody has been reported for use in flow cytometric analysis. Applications Tested: This 4NZBRGO antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at 5 μL (0.5 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 μm post-manufacturing filtered.

GPR109A (also known as HCA2, HM74a, and NIACR1) is a 42kDa G-protein coupled receptor that belongs to a family of three hydroxyl-carboxylic acid receptors, which also include GPR109B and GPR81. GPR109A has two known ligands: niacin and butyrate (or its derivative 3-hydroxybutyrate). The endogenous ligands for GPR109B and GPR81 are 3-hydroxyl-octanoic acid and 2-hydroxy-propanoate (lactate), respectively. GPR109A is expressed on adipocytes, neutrophils, Langerhans cells, keratinocytes, intestinal epithelial cells, and intestinal colonic macrophages. Niacin, also known as nicotinic acid or vitamin B3, is known to improve cholesterol metabolism (LDL/HDL ratio) and reduce inflammation. It has been demonstrated that this effect is mediated through GPR109A. Notably, GPR109A also has an essential role in suppressing inflammation and cancer in the colon. Both GPR109A ligands are produced by colonic commensal bacteria. In mice, GPR109A expression on intestinal epithelial cells and DCs/macrophages contributes to the suppression of colitis and colon cancer. In humans, GPR109A is silenced in colon carcinoma cells by DNA methylation, which increases inflammation in the intestinal tract.

Specifications

• Antigen: GPR109
• Classification: Monoclonal
• Concentration: 5 μL/Test
• Formulation: PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
• Gene Accession No.: Q8TDS4
• Gene Symbols: HCAR2
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 338442
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!
• Form: Liquid

• Applications: Flow Cytometry
• Clone: 4NZBRGO
• Conjugate: PE
• Gene: HCAR2
• Gene Alias: G protein-coupled receptor 109A; G protein-coupled receptor 109B; G protein-coupled receptor HM74a; Gpr109; Gpr109a; Gpr109b; G-protein coupled receptor 109; G-protein coupled receptor 109A; G-protein coupled receptor HM74; G-protein coupled receptor HM74A; HCA2; Hcar2; HM74; HM74a; HM74b; hydroxycarboxylic acid receptor 2; hydroxy-carboxylic acid receptor 2; interferon-gamma inducible gene, Puma-g; mHM74b; Niacin receptor 1; Niacr1; Nicotinic acid receptor; Protein PUMA-G; PUMAG; PUMA-G; PUMA-G. GPR109; putative seven transmembrane spanning receptor
• Host Species: Mouse
• Quantity: 100 Tests
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG1 κ